Effects of Shenyu Buxue Decotion on the Th17 cells and expression of its related IL-6, IL-17 and IL-23 expression in mice with aplastic anemia
ZENG Qing1 LU Haisong1 CHENG Weimin1 HE Ai1 DENG Dejun2 LAI Chunfeng3
1.Department of Hematology, the First Affiliated Hospital of Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530023, China; 2.Graduate School, Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 500001, China;
3.Department of Oncology, Yullin Traditional Chinese Medicine Hospital, Guangxi Zhuang Autonomous Region, Yulin 537000, China
Abstract：Objective To observe the effect of Shenyu Buxue Decotion on Th17 cells and the expression of IL-6, IL-17 and IL-23 in aplastic anemia (AA) model mice, and to explore its curative effect for AA. Methods Eighty clean-class BALB/c mice were divided into control group, model group, Shenyu Buxue Decotion group and Prednisone (positive control) group by random number table. The AA models were established by irradiated with 60CO-γ ray together with intraperitoneal injection of cyclophosphamide (CY) and chloramphenicol (CH). After AA models were successfully established, mice were given to medicines for 4 weeks. The changes of mice peripheral hemogram, Th17 cells and the expression levels of IL-6, IL-17 and IL-23, and the degree of bone marrow hyperplasia under light microscope were observed. Results Compared with the control group, the peripheral hemogram and bone marrow hyperplasia were decreased, while Th17 cells and expression of IL-6, IL-23 and IL-17 were increased in the model group, with statistically significant difference (P < 0.05). Compared with the model group, in the Shenyu Buxue Decotion group, the peripheral hemogram were increased, Th17 cells and the expression of IL-6, IL-23 and IL-17 were decreased, and bone marrow hyperplasia was improved, with statistically significant difference (P < 0.05). Conclusion Shenyu Buxue Decotion can improve the bone marrow hematopoietic function of AA mice, which may due to the inhibition of the T cell immune function related IL-6, IL-17 and IL-23.
曾清1 陆海颂1 程纬民1 何艾1 邓德君2 赖春凤3. 参萸补血方对再生障碍性贫血模型小鼠Th17细胞及其相关因子IL-6、IL-17及IL-23表达水平的影响[J]. 中国医药导报, 2018, 15(12): 13-16.
ZENG Qing1 LU Haisong1 CHENG Weimin1 HE Ai1 DENG Dejun2 LAI Chunfeng3. Effects of Shenyu Buxue Decotion on the Th17 cells and expression of its related IL-6, IL-17 and IL-23 expression in mice with aplastic anemia. 中国医药导报, 2018, 15(12): 13-16.
2018, Vol. 15 
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