脑特异性新生血管抑制因子1在结直肠癌中的表达及其与结直肠癌患者临床病理学特征的关系

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摘要目的探究脑特异性新生血管抑制因子1（BAI1）在结直肠癌中的表达与结直肠癌患者临床病理学特征的关系。方法 2016年5月~2017年7月，购买包含有208个结直肠癌和8个正常结肠组织及其临床信息的组织芯片。查询癌症基因组图谱数据库，内含192例结直肠癌患者的mRNA表达信息。通过免疫组化检测组织芯片正常结肠组织与结直肠癌组织中BAI1表达的情况，检测BAI1表达与结直肠癌患者临床病理特征的关系，采用Kaplan-Meier法和Log-rank秩检验方法进行生存曲线。最后使用Cox回归模型进行单变量分析和多变量分析，判断BAI1对预后的判断价值。结果 BAI1在结直肠癌组织中的含量较正常组织显著上升（P < 0.001）。高BAI1表达与临床分期（P = 0.004）和肿瘤浸润（P = 0.006）相关。癌症基因组图谱（TCGA）中结直肠癌患者的BAI1 mRNA表达进一步提示了较晚的临床分期（P = 0.027）、较严重的肿瘤浸润（P = 0.021）的结直肠癌中BAI1表达水平更高。Kaplan-Meier生存曲线提示BAI1 mRNA高表达的患者比低表达水平的患者总生存期明显减少（P = 0.02）。BAI1 mRNA的高表达是结直肠癌预后的独立影响因素（HR = 2.895，95%CI：1.012~8.281，P = 0.047）。结论 BAI1与结直肠癌的恶性相关。BAI1的高表达可能提示结直肠癌患者的不良预后。

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	魏建昌* 胡鹤* 王强陈转鹏杨平张通曹杰

关键词 ：脑特异性新生血管抑制因子1, 结直肠癌, 临床病理学特征, 独立预后因素

Abstract：Objective To explore the roles of brain-specific angiogenesis inhibitor 1 in colorectal cancer and its correlations with clinicopathological features and prognosis of CRC patients. Methods Fom May 2016 to July 2017, tissue microarray with 208 CRC, 8 normal colon tissues and the clinical information was purchased. The cancer genome atlas (TCGA) dataset is a publicly available dataset with 192 primary CRC patients and the mRNA expressions. BAI1 in TMA of CRC and benign glandular epithelium cells was checked by immunohistochemistry. The exlpore the connection between the express of brain-specific angiogenesis inhibitor 1 with clinicopathological features. Overall survival curve was analyzed by Kaplan-Meier method and Log-rank test. Univariate analysis and multivariate analyses were analyzed with Cox proportional hazards regression to assess the prognostic value of high BAI1 expression for CRC patients. Results BAI1 was localized in the cytoplasm of CRC and benign glandular epithelium cells, showing higher protein levels in CRC tissues compared with normal colon samples (P < 0.001). High BAI1 protein expression was associated with high pathological grade (P = 0.039), advanced clinical stage (P = 0.004) and enhanced tumor invasion (P = 0.006). The cancer genome atlas (TCGA) mRNA expression further showed that BAI1 was upregulated in CRC with advanced clinical stage (P = 0.027) and enhanced tumor invasion (P = 0.021). Kaplan-Meier survival curves revealed that CRC patients with higher BAI1 mRNA levels had shorter overall survival than those with lower expression (P = 0.02). High BAI1 mRNA expression was an independent influence of factor for prognosis of CRC (HR = 2.895; 95%CI: 1.012-8.281; P = 0.047). Conclusion BAI1 ralates to aggressive CRC. High BAI1 expression may predict poor prognosis of CRC patients.

Key words： Brain-specific angiogenesis inhibitor 1 Colorectal cancer Clinicopathological features Independent prognostic factor

基金资助: 广东省广州市科技计划项目（2014y2-00137）。

通讯作者: 曹杰（1964.9-），男，医学博士、管理学博士，教授，主任医师，博士生导师，广州医科大学附属广州市第一人民医院院长，广州消化疾病中心主任；研究方向：胃肠道疾病。

作者简介: *共同第一作者

引用本文:

魏建昌* 胡鹤* 王强陈转鹏杨平张通曹杰. 脑特异性新生血管抑制因子1在结直肠癌中的表达及其与结直肠癌患者临床病理学特征的关系[J]. 中国医药导报, 2018, 15(20): 8-11.
WEI Jianchang* HU He* WANG Qiang CHEN Zhuanpeng YANG Ping ZHANG Tong CAO Jie. Expression of brain-specific angiogenesis inhibitor 1 in colorectal cancer and its correlations with clinicopathological features of colorectal cancer patients. 中国医药导报, 2018, 15(20): 8-11.

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[1] Torre LA，Bray F，Siegel RL，et al. Global cancer statistics，2012 [J]. CA Cancer J Clin，2015，65（2）：87-108 .
[2] Yang P，Wei J，Li W，et al. High expression of growth factor receptor-bound protein 14 predicts poor prognosis for colorectal cancer patients [M]. Biotechnol Lett，2016，38（6）：1043-1047.
[3] He F，Chen H，Yang P，et al. Gankyrin sustains PI3K/GSK-3β/β-catenin signal activation and promotes colorectal cancer aggressiveness and progression [J]. Oncotarget，2016， 7（49）：81 156-81 171.
[4] Mori K，Kanemura Y，Fujikawa H，et al． Brain-specific angiogenesis inhibitor 1（BAI1）is expressed in human cerebral neuronal cells [J]. Neurosci Res，2002，43（1）：69-74.
[5] Cork SM，Van Meir EG. Emerging roles for the BAI1 protein family in the regulation of phagocytosis，synaptogenesis，neurovasculature，and tumor development [J].J Mol Medi，2011，89（8）：743-752.
[6] Nishimori H，Shiratsuchi T，Urano T，et al. A novel brain-specific p53-target gene，BAI1，containing thrombospondin type 1 repeats inhibits experimental angiogenesis [J]. Oncogene，1997，15（18）：2145-2150.
[7] Nishizaki M，Fujiwara T，Tanida T，et al. Recombinant adenovirus expressing wildtypep53 is antiangiogenic：a proposed mechanism forbystander effect [J]. Clin Cancer Res，1999，5（5）：1015-1023.
[8] Hatanaka H，Oshika Y，Abe Y，et al. Vascularization is decreased in pulmonary adenocarcinomaexpressing brain-specific angiogenesis inhibitor 1 （BAI1） [J]. Int J Mol Med，2000，5（2）：181-183.
[9] Miyamoto N，Yamamoto H，Taniguchi H，et al. Differential expression of angiogenesis-related genes in human gastric cancers with and those without high-frequency microsatellite instability [J]. Cancer Lett，2007，254（1）：42-53.
[10] Kaur B，Brat DJ，Calkins CC，et al. Brain angiogenesis inhibitor 1 is differentially expressed in normal brain and glioblastoma independently of p53 expression [J]. Am J Pathol，2003，162（1）：19-27.
[11] Nam DH，Park K，Suh YL，et al. Expression of VEGF and brain specific angiogenesis inhibitor-1 in glioblastoma：prognostic significance [J]. Oncol Rep，2004，11（4）：863-869.
[12] Kang X，Xiao X，Harata M，et al. Antiangiogenic activity of BAI1 in vivo：implications for gene therapy of human glioblastomas [J]. Cancer Gene Ther，2006，13（4）：385-392.
[13] Izutsu T，Konda R，Sugimura J，et al. Brain-Specific Angiogenesis Inhibitor 1 is a Putative Factor for Inhibition of Neovascular Formation in Renal Cell Carcinoma [J]. J Urol，2011，185（6）：2353-2358.
[14] Kudo S，Konda R，Obara W，et al. Inhibition of tumor growth through suppression of angiogenesis by brain-specific angiogenesis inhibitor 1 gene transfer in murine renal cell carcinoma [J]. Oncol Rep，2007，18（4）：785-791.
[15] Meisen WH，Dubin S，Sizemore ST，et al. Changes in BAI1 and Nestin expression are prognostic indicators for survival and metastases in Breast Cancer and Provide Opportunities for Dual Targeted Therapies [J]. Mol Cancer Ther，2015，14（1）：307-314.
[16] Van Meir EG，Polverini PJ，Chazin VR，et al. Release of aninhibitor of angiogenesis upon induction of wild type p53 expression in glioblastoma cells [J]. Nat Genet，1994， 8（2）：171-176.
[17] Monk KR，Naylor SG，Glenn TD，et al. A G protein-coupled receptor is essential for Schwann cells to initiate myelination [J]. Science，2009，325（5946）：1402-11405.
[18] Sherman DL，Brophy PJ. Mechanisms of axon ensheathment and myelin growth [J]. Nat Rev Neurosci，2005，6（9）：683-690.
[19] Fukushima Y，Oshika Y，Tsuchida T，et al. Brain-specific angiogenesis inhibitor 1 expression is inverselycorrelated with vascularity and distant metastasis of colorectal cancer [J]. Int J Oncol，1998，13（5）：967-970.
[20] Yoshida Y，Oshika Y，Fukushima Y，et al. Expression of angiostatic factors in colorectal cancer [J]. Int J Oncol，1999， 15（6）：1221-1225.