MMP2、IGF2BP2及HMGA2在侵入性胎盘组织中的表达

doi:10.20047/j.issn1673-7210.2022.33.20

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摘要目的探讨基质金属蛋白酶2（MMP2）、胰岛素样生长因子2 mRNA结合蛋白2（IGF2BP2）及高迁移率族蛋白A2（HMGA2）在侵入性胎盘组织中的表达。方法选取2021年3月至12月郑州大学第二附属医院产检分娩并确诊为侵入性胎盘的23例孕产妇为侵入性胎盘组，同期正常妊娠的29名孕产妇为对照组。采用Western blot和实时定量PCR检测两组胎盘组织中MMP2、IGF2BP2、HMGA2的表达。结果侵入性胎盘组MMP2、IGF2BP2、HMGA2蛋白及mRNA表达水平高于对照组，差异有统计学意义（P ＜ 0.05）。结论胎盘组织中MMP2、IGF2BP2及HMGA2表达升高可能与侵入性胎盘疾病的发生有关。

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	叶芳兵朱宝菊▲

关键词 ：侵入性胎盘, 胰岛素样生长因子2mRNA结合蛋白2, 高迁移率族蛋白A2, 基质金属蛋白酶2

Abstract：Objective To investigate the expressions of matrix metalloproteinase 2 (MMP2), insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2), and high mobility group box A2 (HMGA2) in invasive placental tissue. Methods From March to December 2021, 23 pregnant women diagnosed with invasive placenta in the Second Affiliated Hospital of Zhengzhou University were selected as invasive placenta group, and 29 pregnant women with normal pregnancy in the same period were selected as control group. Western blot and real-time PCR were used to detect the expressions of MMP2, IGF2BP2, and HMGA2 in placental tissues of the two groups. Results The protein and mRNA expression levels of MMP2, IGF2BP2, and HMGA2 in invasive placenta group were higher than those in control group, and the differences were statistically significant (P ＜ 0.05). Conclusion The elevated expression of MMP2, IGF2BP2, and HMGA2 in placental tissue may be related to the occurrence of invasive placental tissue.

Key words： Invasive placenta Insulin-like growth factor 2 mRNA-binding protein 2 High mobility group box A2 Matrix metalloproteinase 2

基金资助:河南省科技攻关项目（172102310085）。

通讯作者: ▲通讯作者

引用本文:

叶芳兵朱宝菊▲. MMP2、IGF2BP2及HMGA2在侵入性胎盘组织中的表达[J]. 中国医药导报, 2022, 19(33): 92-95.
YE Fangbing ZHU Baoju▲. Expression of MMP2, IGF2BP2, and HMGA2 in invasive placental tissue. 中国医药导报, 2022, 19(33): 92-95.

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[1] Jauniaux E，Bhide A，Kennedy A，et al. FIGO consensus guidelines on placenta accreta spectrum disorders：Prenatal diagnosis and screening [J]. Int J Gynaecol Obstet，2018，140（3）：274-280. DOI：10.1002/ijgo.12408.
[2] Silver RM，Branch DW. Placenta Accreta Spectrum [J]. N Engl J Med，2018，378（16）：1529-1536. DOI：10.1056/ NEJMcp1709324.
[3] Kapoor H，Hanaoka M，Dawkins A，et al. Review of MRI imaging for placenta accreta spectrum：Pathophysiologic insights，imaging signs，and recent developments [J]. Placenta，2021，104：31-39. DOI：10.1016/j.placenta.2020.11.004.
[4] Jauniaux E，Bunce C，Gr?覬nbeck L，et al. Prevalence and main outcomes of placenta accreta spectrum：a systematic review and meta-analysis [J]. Am J Obstet Gynecol，2019， 221（3）：208-218. DOI：10.1016/j.ajog.2019.01.233.
[5] Wehrum MJ，Buhimschi IA，Salafia C，et al. Accreta complicating complete placenta previa is characterized by reduced systemic levels of vascular endothelial growth factor and by epithelial-to-mesenchymal transition of the invasive trophoblast [J]. Am J Obstet Gynecol，2011，204（5）：411.e1- 411.e11. DOI：10.1016/j.ajog.2010.12.027.
[6] Ali MAM，Garcia-Vilas JA，Cromwell CR，et al. Matrix metalloproteinase-2 mediates ribosomal RNA transcription by cleaving nucleolar histones [J]. FEBS J，2021，288（23）：6736-6751. DOI：10.1111/febs.16061.
[7] Cancemi P，Buttacavoli M，Roz E，et al. Expression of Alpha-Enolase（ENO1），Myc Promoter-Binding Protein-1（MBP-1）and Matrix Metalloproteinases（MMP-2 and MMP-9）Reflect the Nature and Aggressiveness of Breast Tumors [J]. Int J Mol Sci，2019，20（16）：3952. DOI：10.3390/ ijms 20163952.
[8] Myers JE，Merchant SJ，Macleod M，et al. MMP-2 levels are elevated in the plasma of women who subsequently develop preeclampsia [J]. Hypertens Pregnancy，2005，24（2）：103- 115. DOI：10.1081/PRG-200059836.
[9] Ding J，Huang F，Wu G，et al. MiR-519d-3p suppresses invasion and migration of trophoblast cells via targeting MMP-2 [J]. PLoS One，2015，10（3）：e0120321. DOI：10.1371/journal.pone.0120321.
[10] Kocarslan S，Inceb?覦y?覦k A，Guldur ME，et al. What is the role of matrix metalloproteinase-2 in placenta percreta? [J]. J Obstet Gynaecol Res，2015，41（7）：1018-1022. DOI：10.1111/jog.12667.
[11] Huang H，Weng H，Sun W，et al. Recognition of RNA N6- methyladenosine by IGF2BP proteins enhances mRNA stability and translation [J]. Nat Cell Biol，2018，20（3）：285-295. DOI：10.1038/s41556-018-0045-z.
[12] Xu X，Yu Y，Zong K，et al. Up-regulation of IGF2BP2 by multiple mechanisms in pancreatic cancer promotes cancer proliferation by activating the PI3K/Akt signaling pathway [J]. J Exp Clin Cancer Res，2019，38（1）：497. DOI：10.1186/ s13046-019-1470-y.
[13] Dahlem C，Barghash A，Puchas P，et al. The Insulin-Like Growth Factor 2 mRNA Binding Protein IMP2/IGF2BP2 is Overexpressed and Correlates with Poor Survival in Pancreatic Cancer [J]. Int J Mol Sci，2019，20（13）：3204. DOI：10.3390/ijms20133204.
[14] Yang Y，Liu X，Cheng L，et al. Tumor Suppressor microRNA-138 Suppresses Low-Grade Glioma Development and Metastasis via Regulating IGF2BP2 [J]. Onco Targets Ther，2020，13：2247-2260. DOI：10.2147/OTT.S232795.
[15] Liu HB，Muhammad T，Guo Y，et al. RNA-Binding Protein IGF2BP2/IMP2 is a Critical Maternal Activator in Early Zygotic Genome Activation [J]. Adv Sci（Weinh），2019，6（15）：1900295. DOI：10.1002/advs.201900295.
[16] Wu L，Song WY，Xie Y，et al. miR-181a-5p suppresses invasion and migration of HTR-8/SVneo cells by directly targeting IGF2BP2 [J]. Cell Death Dis，2018，9（2）：16. DOI：10.1038/s41419-017-0045-0.
[17] Yang Q，Ma Y，Liu Y，et al. MNSFβ regulates placental development by conjugating IGF2BP2 to enhance trophoblast cell invasiveness [J]. Cell Prolif，2021，54（12）：e13145. DOI：10.1111/cpr.13145.
[18] Chen CH，Hsieh YC，Yang PM，et al. Dicoumarol suppresses HMGA2-mediated oncogenic capacities and inhibits cell proliferation by inducing apoptosis in colon cancer [J]. Biochem Biophys Res Commun，2020，524（4）：1003- 1009. DOI：10.1016/j.bbrc.2020.01.147.
[19] Mansoori B，Duijf PHG，Mohammadi A，et al. Overexpression of HMGA2 in breast cancer promotes cell proliferation，migration，invasion and stemness [J]. Expert Opin Ther Targets，2020，24（3）：255-265. DOI：10.1080/14728222. 2020.1736559.
[20] Hawsawi O，Henderson V，Burton LJ，et al. High mobility group A2 （HMGA2） promotes EMT via MAPK pathway in prostate cancer [J]. Biochem Biophys Res Commun，2018， 504（1）：196-202. DOI：10.1016/j.bbrc.2018.08.155.
[21] Zhang S，Mo Q，Wang X. Oncological role of HMGA2（Review）[J]. Int J Oncol，2019，55（4）：775-788. DOI：10.3892/ ijo.2019.4856.
[22] Wei L，Liu X，Zhang W，et al. Overexpression and oncogenic function of HMGA2 in endometrial serous carcinogenesis [J]. Am J Cancer Res，2016，6（2）：249-259.
[23] Bamberger AM，Makrigiannakis A，R?觟ser K，et al. Expression of the high-mobility group protein HMGI（Y）in human trophoblast：potential role in trophoblast invasion of maternal tissue [J]. Virchows Arch，2003，443（5）：649-654. DOI：10.1007/s00428-003-0892-1.
[24] Genbacev O，Larocque N，Ona K，et al. Integrin α4-positive human trophoblast progenitors：functional characterization and transcriptional regulation [J]. Hum Reprod，2016， 31（6）：1300-1314. DOI：10.1093/humrep/dew077.
[25] Genbacev O，Donne M，Kapidzic M，et al. Establishment of human trophoblast progenitor cell lines from the chorion [J]. Stem Cells，2011，29（9）：1427-1436. DOI：10.1002/stem. 686.
[26] Abi Habib W，Brioude F，Edouard T，et al. Genetic disruption of the oncogenic HMGA2-PLAG1-IGF2 pathway causes fetal growth restriction [J]. Genet Med，2018，20（2）：250-258. DOI：10.1038/gim.2017.105.
[27] Burchardt L，Gottlieb A，Helmke BM，et al. Expression patterns of HMGA2 in the placenta during pregnancy [J]. medRxiv，2020，12（7）：92-111.