Expression and significance of microRNA-221 in non-small cell lung cancer
HE Jiayong1 YANG Chenchen2 XU Qian2
1.NO.1 Department of Surgery, Staff-worker Hospital of Urumqi Petrochemical Subsidiary China National Petroleum, Xinjiang Uygur Autonomous Region, Urumqi 830019, China;
2.Teaching Office of Immunology, College of Preclinical Medicine, Cancer Hospital Affiliated of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830011, China
Abstract：Objective To investigate the expression and significance of microRNA-221 in non-small cell lung cancer (NSCLC). Methods A total of 53 NSCLC cases′ clinical material were collected from Cancer Hospital Affiliated of Xinjiang Medical University from January 2010 to January 2011, and all the cases accepted pulmonary lobectomy and systematic mediastinal lymphadenectomy. Levels of miRNA-221 in the NSCLC and adjacent tissues were examined by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). The relationship between expression level of miRNA-221 and clinic pathological parameters were analyzed. Kaplan-Meier was used to analyze the relationship between miRNA-221 expression and overall survival (OS). Results The relative expression of miRNA-221 in NSCLC tissues was (0.0234±0.0002), and it was (0.0095±0.0003) in adjacent tissues. The relative expression of miRNA-221 in NSCLC tissues was higher than that in adjacent tissues (P ＜ 0.05). There were 31 cases having relative higher expression. The level of miRNA-221 was significantly higher in TNM stage Ⅲ than that in TNM stage Ⅰ-Ⅱ(P ＜ 0.05). Kaplan-Meir survival analysis results manifested that the median OS of patients with miRNA-221 high expression was 36.4 months. There was no significant difference in OS between miRNA-221 low expression and high expression patients(P ＞ 0.05). Conclusion miRNA-221 is a potential molecular marker for the prognosis of lung cancer, which may be involved in the development and progression of NSCLC.
2018, Vol. 15 
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