Expression and clinical significance of serum miR-29a and miR-143-3p in patients with polycystic ovary syndrome
FANG Xiuli1 WANG Daying1 HUANG Xiumin1 CHAO Pengli2 SHE Jingrong3 WEI Zhuohua3
1.Department of Obstetrics and Gynecology, Zhongshan Hospital Affiliated to Xiamen University, Fujian Province, Xiamen 361004, China; 2.Department of Clinical Laboratory, Zhongshan Hospital Affiliated to Xiamen University, Fujian Province, Xiamen 361004, China; 3.Department of Clinical Medicine, Xiamen University Medical School, Fujian Province, Xiamen 361004, China
Abstract：Objective To analyze the relationship between serum microRNA (miRNA)-29a and miR-143-3p expression and obesity, glucolipid metabolism, and insulin resistance (IR) in patients with polycystic ovary syndrome (PCOS). Methods A total of 86 patients with PCOS admitted to Zhongshan Hospital Affiliated to Xiamen University (hereinafter referred to as “our hospital”) from June 2019 to June 2022 were selected as the PCOS group, and 49 healthy volunteers with physical examination in our hospital during the same period were selected as the control group. PCOS patients were divided into the obese group (36 cases) and the non-obese group(50 cases) according to body mass index (BMI), and they were divided into the IR group (61 cases) and the non-IR group (25 cases) according to homeostasis model assessment (HOMA)-IR. Serum miR-29a, miR-143-3p expression and obesity indicators (BMI, waist-to-hip ratio [WHR]), glucolipid metabolism indicators (fasting blood glucose [FBG], fasting insulin [FINS], total cholesterol [TC], triacylglycerol [TG], high-density lipoprotein cholesterol [HDL-C], and low-density lipoprotein cholesterol [LDL-C]) and HOMA-IR of all groups were compared; the correlation between serum miR-29a and miR-143-3p expression and obesity indicators, glucolipid metabolism indicators, and HOMA-IR in PCOS patients were analyzed. Results BMI, WHR, FBG, FINS, HOMA-IR, TC, TG, LDL-C, miR-29a, and miR-143-3p in PCOS group were higher than those in control group, and HDL-C was lower than that in the control group (P＜0.05). Serum miR-29a and miR-143-3p expression in the obese group were higher than those in the non-obese group (P＜0.05). Serum miR-29a and miR-143-3p expression in the IR group were higher than those in the non-IR group (P＜0.05). Serum miR-29a and miR-143-3p expression in PCOS patients were positively correlated with BMI, WHR, FBG, FINS, HOMA-IR, TC, TG, and LDL-C (r/rs＞0, P＜0.001) and HDL-C was negatively correlated (r＜0, P＜0.001). Conclusion High expression of serum miR-29a and miR-143-3p in PCOS patients is closely associated with obesity, glucose and lipid metabolism, and IR.
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2023, Vol. 20 
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