Mechanism of persistent low back pain after intervertebral space infection
YANG Huan1 SONG Hongxing2▲ CUI Lei3 YUAN Wei2
1.Beijing Shijitan Hospital, Capital Medical University Beijing 100038, China;
2.Department of Orthopaedics Surgery, Beijing Shijitan Hospital, Capital Medical University Beijing 100038, China;
3.Department of Plastic Surgery, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China
Abstract：Objective To Discus on the Staphylococcus aureus supernatant of intervertebral disc nucleus pulposus cells proteoglycans (ACAN), type Ⅱ collagen (COL Ⅱ). Methods Cultured SD rat intervertebral disc nucleus pulposus cells were isolated in vitro and treated with Staphylococcus aureus supernatant at different concentrations (0%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%) to simulate the course of intervertebral space infection. CCK-8 was used to detect the proliferation activity of the second generation nucleus pulposus (P2) cells. RT-PCR was used to detect the gene expression of the second generation nucleus pulposus cells. Results This study found that the supernatant fluid of Staphylococcus aureus that accelerate nucleus pulposus cells to divide, stimulate ACAN, COL Ⅱ expression decreased (P ＜ 0.05), the supernatant fluid concentration > 20%, can significantly inhibit extracellular matrix synthesis of nucleus pulposus. Conclusion The results showed that Staphylococcus aureus supernatant could inhibit the growth of nucleus pulposus cells in a concentration dependent manner. Stimulate ACAN, COL Ⅱ expression decrease may be the cause of intervertebral infection after continuous low back pain.
杨欢1 宋红星2▲ 崔磊3 原威2. 椎间隙感染后持续腰背痛的机制研究[J]. 中国医药导报, 2019, 16(13): 8-11.
YANG Huan1 SONG Hongxing2▲ CUI Lei3 YUAN Wei2. Mechanism of persistent low back pain after intervertebral space infection. 中国医药导报, 2019, 16(13): 8-11.
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2019, Vol. 16 
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