Abstract：Objective To study the effects of cinobufacini combined with Cisplatin on cell viability, apoptosis and cell cycle distribution of breast cancer MDA-MB-231 cells. Methods Breast cancer MDA-MB-231 cells were cultured in vitro. CCK-8 assay was used to detect the effect of cinobufacini combined with Cisplatin on cell viability. Scratch test was performed to detect the effect of cinobufacini combined with Cisplatin on cell migration. Flow cytometry was used to analyze the effects of cinobufacini combined with Cisplatin on the apoptosis rate and cell cycle distribution of breast cancer cells. Results CCK-8 results showed that compared with the control group, the cell viability in the cinobufacini group, the Cisplatin group and the cinobufacini+Cisplatin group was decreased, the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). The scratch results showed that the migration ability of the cinobufacini+Cisplatin group was significantly decreased compared with the control group, the difference was highly statistically significant (P ＜ 0.01). Flow cytometry showed that compared with the control group, the proportion of G1 phase in the cinobufacini+Cisplatin group increased significantly and the apoptosis was more obvious, the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). Conclusion Cinobufacini combined with Cisplatin shows a synergic effect in inhibiting MDA-MB-231 cells, which may be related to the induction of cell cycle arrest at G1 phase, inhibition of cell migration and promotion of cell apoptosis.
肖聪* 张懿敏* 孙圣荣. 华蟾素联用顺铂对乳腺癌MDA-MB-231细胞活力、凋亡及周期分布的影响[J]. 中国医药导报, 2019, 16(21): 12-16.
XIAO Cong* ZHANG Yimin* SUN Shengrong. Effect of cinobufacini combined with Cisplatin on cell viability, apoptosis and cell cycle distribution of MDA-MB-231 cells. 中国医药导报, 2019, 16(21): 12-16.
2019, Vol. 16 
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