Macrotranscriptome analysis of subgingival plaque in patients with chronic periodontitis
XIAO Weizhuo1* ZHANG Long2* WANG Xiaodong3 ZHANG Baorong1,3
1.School of Stomatology, Weifang Medical University, Shandong Province, Weifang 261000, China;
2.Department of Stomatology, University of Chinese Academy of Sciences, Shenzhen Hospital, Guangdong Province, Shenzhen 518000, China;
3.Department of Stomatology, Aviation General Hospital, Beijing 100012, China
Abstract：Objective To analyze the transcriptome of subgingival plaque in chronic periodontitis and explore the changes of subgingival plaque community in chronic periodontitis. Methods From January 2017 to January 2018, 7 cases of subgingival plaque patients diagnosed as chronic periodontitis in the Department of Stomatology of Aviation General Hospital and meeting the inclusion conditions were selected. Relevant RNA was extracted and trace RNA was used to build a database. High-throughput second-generation sequencing technology was used to analyze the macrotranscriptome and the gene expression of related bacteria was observed. Results In the subgingival plaque of chronic periodontitis, the gene sequence of Venn treponema was highly expressed. Among the homologous protein families closely related to periodontitis, the most expressed gene sequences were related to bacterial translation. Among the active carbohydrate enzyme-related genes, the most expressed ones were the sequences of carbohydrate related domain genes and glycoside hydrolytic enzyme genes. Conclusion Macrotranscriptome sequencing analysis is conducted to understand the gene expression of different bacteria in subgingival plaque of chronic periodontitis patients and the gene expression of related bacteria physiological metabolism, so as to propose new research methods and opinions on the pathogenicity of dental plaque, and deepen the understanding of the pathogenicity of plaque.
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2019, Vol. 16 
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