Protective effect and mechanism of Chinese eaglewood extract on gastric ulcer induced by ethanol in rats
MA Lin1 ZHANG Xin2 CHEN Huanxin2 ZHOU Hailing1 LI Jiaqi2 WANG Lingli2
1.Department of Pharmacy, the Second Clinical Medical College, Guangzhou University of Chinese Medicine, Guangdong Province, Guangzhou 510120, China; 2.Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangdong Province, Guangzhou 510006, China
Abstract:Objective To study the protective effect of Chinese eaglewood extract on gastric ulcer tissue and its mechanism. Methods Sixty SPF Sprague-Dawley (SD) male rats were divided into normal group (not to deal with), model group (equal volume of normal saline), positive control group (Lansoprazol 0.003 g/mL), Chinese eaglewood extract low (0.4 g/mL), medium (0.8 g/mL) and high (1.6 g/mL) dose groups by random number table method, the dosage of each group was 1 mL/100 g, with 10 rats in each group. The acute gastric mucosal injury model was established by intragastric administration of anhydrous ethanol 1 mL. The positive control group and Chinese eaglewood extract groups were given intragastric administration once a day for 7 days. The contents of Bcl-2 and Bax were examined by immunohistochemical method. The kit was used to detect the content of oxidative factor malondialdehyde (MDA) and the activity of antioxidant factor superoxide dismutase (SOD) and trace reduced glutathione (GSH) in rat serum. Results Compared with the normal group, the expression of Bcl-2 protein in the gastric tissue of the model group decreased, and the expression of Bax protein increased (P < 0.05). Compared with the model group, the expression of Bcl-2 in the gastric tissue of Chinese eaglewood extract groups increased, and the expression of Bax protein decreased (P < 0.05). Compared with the model group, the activity of SOD and GSH of Chinese eaglewood extract groups increased and the content of MDA decreased (P < 0.05). Conclusion Chinese eaglewood extract can improve the gastric ulcer induced by absolute ethanol through increasing the expression of Bcl-2 protein in gastric tissue, reducing the expression of Bax protein, improving the activity of serum SOD and GSH and decreasing the content of MDA.
马麟1 张昕2 陈焕鑫2 周海玲1 李嘉琪2 王羚郦2. 沉香提取液对乙醇致大鼠胃溃疡的保护作用及机制[J]. 中国医药导报, 2019, 16(24): 15-18.
MA Lin1 ZHANG Xin2 CHEN Huanxin2 ZHOU Hailing1 LI Jiaqi2 WANG Lingli2. Protective effect and mechanism of Chinese eaglewood extract on gastric ulcer induced by ethanol in rats. 中国医药导报, 2019, 16(24): 15-18.