补肾活血颗粒对去卵巢小鼠心脏巨噬细胞M1/M2极化的影响

摘要
图/表
参考文献(20)
相关文章 (1)

全文: PDF (668 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的探讨补肾活血颗粒是否能够通过调控巨噬细胞M1/M2极化治疗去卵巢小鼠心脏微血管病变。方法按照随机数字表法将30只SPF级8周龄C57BL/6小鼠分为假手术组、模型组和治疗组。制备去卵巢小鼠模型8周后，治疗组给予补肾活血颗粒（含生药6 g/mL），灌胃8周，1次/d；假手术组和模型组给予同体积生理盐水，灌胃8周，1次/d。通过HE染色、免疫荧光染色和实时定量PCR等方法检测补肾活血颗粒对心脏组织结构、超氧阴离子、CD31、CD68和白细胞介素（IL）-1β、肿瘤坏死因子（TNF）-α、IL-10、血管内皮生长因子（VEGF）的mRNA表达的影响。结果与假手术组比较，模型组心脏组织中IL-1β mRNA、TNF-α mRNA、超氧阴离子、CD68表达明显增加（P ＜ 0.05），CD31、IL-10 mRNA表达明显减少（P ＜ 0.05）。与模型组比较，治疗组小鼠心脏组织中IL-1β mRNA、TNF-α mRNA、超氧阴离子明显降低（P ＜ 0.05），CD31、IL-10 mRNA表达明显增多（P ＜ 0.05），但两组CD68表达差异无统计学意义（P ＞ 0.05）。结论补肾活血颗粒可能通过调控巨噬细胞M1/M2极化治疗去卵巢小鼠心脏微血管病变。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	贾承明马静陈昌波曹亮李颖琪吴雷涛

关键词 ：补肾活血颗粒, 巨噬细胞极化, 心脏微血管病变, 去卵巢

Abstract：Objective To explore whether Bushen Huoxue Granules could cure cardiac microvascular dysfunction by regulating M1/M2 macrophage polarization in female ovariectomized mice. Methods According to random number table, 30 SPF level and 8 weeks old C57BL/6 mice were divided into sham group, model group and treatment group. Eight weeks after ovariectomy, treatment group was given Bushen Huoxue Granules (containing crude drugs 6 g/mL) by gavage, once a day, for eight weeks. Sham group and model group were given the same volume of physiological saline. HE staining, immunofluorescence staining and real-time quantitative PCR were used to detect the influences of Bushen Huoxue Granules on organization structures of heart tissue, the ultra oxygen anion, platelets endothelial cell adhesion molecules (CD31), CD68, and the mRNA expression of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-10 and vascular endothelial growth factor (VEGF). Results Compared with the sham group, the expression of IL-1β mRNA, TNF-α mRNA, ultra oxygen anion, CD68 cells in model group were increased (P ＜ 0.05), while the expression of CD31, IL-10 mRNA were decreased (P ＜ 0.05). Compared with the model group, the expression of IL-1β mRNA, TNF-α mRNA, ultra oxygen anion of heart tissue in the treatment group were decreased (P ＜ 0.05), while the expression of CD31 and IL-10 mRNA were increased (P ＜ 0.05). No significant difference was found with the expression of CD68 between the model group and treatment group (P ＞ 0.05). Conclusion Bushen Huoxue Granules may cure cardiac microvascular dysfunction by regulating M1/M2 macrophage polarization in ovariectomized mice.

Key words： Bushen Huoxue Granules Macrophage polarization Cardiac microvascular dysfunction Ovariectomized

基金资助:中国医药教育协会孙思邈中医药研究专项课题（2016SKT-M001）；
陕西省中医药管理局重大病种中医药创新计划项目（2015-GXB-02）；
陕西省西安市科学技术局社会发展引导计划课题[SF1511（7）]。

通讯作者: 马静（1966.2-），女，博士，副教授，副主任医师；研究方向：中西医结合防治心血管病。

作者简介: 贾承明（1992.6-），男，第四军医大学第一附属医院2014级中西医结合临床专业在读硕士研究生；研究方向：中西医结合防治心血管病。

引用本文:

贾承明马静陈昌波曹亮李颖琪吴雷涛 . 补肾活血颗粒对去卵巢小鼠心脏巨噬细胞M1/M2极化的影响[J]. 中国医药导报, 2017, 14(14): 29-32.
JIA Chengming MA Jing CHEN Changbo CAO Liang LI Yingqi WU Leitao. Influences of Bushen Huoxue Granules on M1/M2 macrophage polarization in the heart of ovariectomized mice. 中国医药导报, 2017, 14(14): 29-32.

链接本文:

http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2017/V14/I14/29

[1] 刘腾飞，丁志坚.微循环阻力指数的研究进展[J].中国医药导报，2015，12（7）：33-36.
[2] Meneguz MR，Costa JJ，Costa RA，et al. Embolic protection devices in percutaneous coronary intervention [J]. Minerva Cardioangiol，2016，64（3）：354-365.
[3] Vitale C，Rosano GM，Kaski JC. Role of coronary microvascular dysfunction in takotsubo cardiomyopathy [J]. Circ J，2016，80（2）：299-305.
[4] Akasaka T，Hokimoto S，Sueta D，et al. Sex differences in the impact of CYP2C19 polymorphisms and low-grade inflammation on coronary microvascular disorder [J]. Am J Physiol Heart Circ Physiol，2016，310（11）：H1494-H1500.
[5] Pop D，Dadarlat A，Zdrenghea D. Novel cardiovascular risk markers in women with ischaemic heart disease [J]. Cardiovasc J Afr，2014，25（3）：137-141.
[6] O'Shea JJ，Paul WE. Mechanisms underlying lineage commitment and plasticity of helper CD4+ T cells [J]. Science，2010，327（5969）：1098-1102.
[7] 汶医宁，马静，史凡凡，等.补肾活血颗粒治疗绝经后微血管性心绞痛的疗效及其对血清NO、ET-1的影响[J].陕西中医，2016，37（8）：987-988.
[8] 韩为华，乔黎焱，刘镘利，等.二仙汤加味对去卵巢大鼠心肌NF-κBp50、IκBα的影响[J].中国中医急症，2011，20（9）：1440-1441.
[9] 张玉芬，王玉珍，赵洁，等.补肾活血汤对去卵巢大鼠心脏微血管内皮细胞形态及血清E2、ET、NO、PGI2、TXA2含量的影响[J].现代生物医学进展，2014，14（31）：6044-6049.
[10] Lizotte E，Grandy SA，Tremblay A，et al. Expression，distribution and regulation of sex steroid hormone receptors in mouse heart [J]. Cell Physiol Biochem，2009，23（1/2/3）：75-86.
[11] 杜纳纳，许丹丹，黄莉莉.去卵巢和性成熟期ICR雌性小鼠动情周期的观察[J].中国病理生理杂志，2015，31（12）：2301-2304.
[12] Mu LX，Zhu L，Zhao AH，et al. Study on extraction and purification of polysaccharides from Astragalus membrana-ceus [J]. J Chin Med Mater，2009，32（11）：1741-1745.
[13] Wu XL，Zheng B，Jin LS，et al. Chinese medicine Tongxinluo reduces atherosclerotic lesion by attenuating oxidative stress and inflammation in microvascular endothelial cells [J]. Int J Clin Exp Pathol，2015，8（6）：6323-6333.
[14] Ivashkiv LB. Epigenetic regulation of macrophage polarization and function [J]. Trends Immunol，2013，34（5）：216-223.
[15] Qin X，Qiu C，Zhao L. Lysophosphatidylcholine perpetuates macrophage polarization toward classically activated phenotype in inflammation [J]. Cell Immunol，2014，289（1/2）：185-190.
[16] Mills CD. M1 and M2 Macrophages：Oracles of Health and Disease [J]. Crit Rev Immunol，2012，32（6）：463-488.
[17] Mills CD，Ley K. M1 and M2 macrophages：the chicken and the egg of immunity [J]. J Innate Immun，2014，6（6）：716-726.
[18] Palmer JA，Abberton KM，Mitchell GM，et al. Macrophage phenotype in response to implanted synthetic scaffolds：an immunohistochemical study in the rat [J]. Cells Tissues Organs，2014，199（2/3）：169-183.
[19] Han J，Shuvaev VV，Muzykantov VR. Catalase and superoxide dismutase conjugated with platelet-endothelial cell adhesion molecule antibody distinctly alleviate abnormal endothelial permeability caused by exogenous reactive oxygen species and vascular endothelial growth factor [J]. J Pharmacol Exp Ther，2011，338（1）：82-91.
[20] Ellinsworth DC. Arsenic，reactive oxygen，and endothelial dysfunction [J]. J Pharmacol Exp Ther，2015，353（3）：458-464.