细胞因子信号转导负调控蛋白2在变应性鼻炎中的表达及临床意义

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摘要目的观察细胞因子信号转导负调控蛋白2（SOCS2）在变应性鼻炎（AR）鼻黏膜组织中的表达及临床意义。方法采用实时定量聚合酶链反应检测南昌大学第一附属医院2018年2～7月行鼻中隔偏曲或翼管神经切断术的20例AR患者（AR组）和15例单纯鼻中隔偏曲患者（对照组）下鼻甲黏膜中的SOCS2、γ干扰素（IFN-γ）、FOXP3、白细胞介素（IL）-4、IL-5、IL-13 mRNA的表达水平。采用免疫组化法检测两组患者鼻黏膜组织中SOCS2蛋白表达情况。采用视觉模拟评分（VAS）评估患者的主观症状。结果 SOCS2在AR组和对照组中均有免疫组化表达；AR组SOCS2平均吸光度值显著低于对照组，差异有统计学意义（P ＜ 0.05）。AR组的SOCS2、IFN-γ和FOXP3相对表达量显著低于对照组，IL-5、IL-13、IL-4相对表达量显著高于对照组，差异有统计学意义（P ＜ 0.05）。AR患者SOCS2相对表达与IL-4、IL-5、IL-13、VAS总分相对表达呈负相关（r = -0.66、-0.50、-0.51、-0.65，P < 0.05），与FOXP3相对表达呈正相关（r = 0.67，P < 0.05），与IFN-γ相对表达无相关性（P > 0.05）。结论 AR患者鼻黏膜组织中SOCS2降低，与AR多种细胞因子相关，提示SOCS2在AR的发病机制中可能起关键作用。

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	刘海涛1 罗庆1 范立1 杨甜2▲

关键词 ：变应性鼻炎, 细胞因子信号转导负调控蛋白2, 细胞因子, 炎症

Abstract：Objective To observe the expression of negative regulatory protein 2 (SOCS2) of cytokine signal transduction in the nasal mucosa of allergic rhinitis (AR) and its clinical significance. Methods The expression levels of SOCS2, interferon-γ (IFN-γ), FOXP3, interleukin (IL)-4, IL-5 and IL-13 mRNA in inferior turbinate mucosa of 20 AR patients (AR group) and 15 patients with simple deviation of nasal septum (control group) underwent nasal septum deviation or pterygoid canal neurotomy from February to July 2018 in the First Affiliated Hospital of Nanchang University were detected by real-time quantitative PCR. Immunohistochemical method was used to detect the expression of SOCS2 protein in nasal mucosa of two groups of patients. Visual analogue scale (VAS) was used to evaluate the subjective symptoms of patients. Results The expression of SOCS2 was immunohistochemistry in AR group and control group. The average absorbance value of SOCS2 in AR group was significantly lower than that in control group, the difference was statistically significant (P < 0.05). The relative expression of SOCS2, IFN-γ and FOXP3 in AR group was significantly lower than that in control group, while the relative expression of IL-5, IL-13 and IL-4 in AR group was significantly higher than that in control group, the differences were statistically significant (P < 0.05). In AR patients, the relative expression of SOCS2 was negatively correlated with the total score of IL-4, IL-5, IL-13 and VAS (r = -0.66, -0.50, -0.51, -0.65, P < 0.05), positively correlated with the relative expression of FOXP3 (r = 0.67, P < 0.05), and not correlated with the relative expression of IFN-γ (P > 0.05). Conclusion The decrease of SOCS2 in the nasal mucosa of AR patients is related to many cytokines of AR, suggesting that SOCS2 may play a key role in the pathogenesis of AR.

Key words： Allergic rhinitis Cytokine signal transduction negative regulatory protein 2 Cytokine Inflammation

基金资助:江西省教育厅科学技术研究一般项目（161455）；
江西省卫生计生委科技计划项目（20163002）。

通讯作者: ▲通讯作者

引用本文:

刘海涛1 罗庆1 范立1 杨甜2▲. 细胞因子信号转导负调控蛋白2在变应性鼻炎中的表达及临床意义[J]. 中国医药导报, 2019, 16(33): 19-22.
LIU Haitao1 LUO Qing1 FAN Li1 YANG Tian2▲. Expression and clinical significance of negative regulatory protein 2 of cytokine signal transduction in allergic rhinitis. 中国医药导报, 2019, 16(33): 19-22.

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