Inhibitory effect of ganoderma lucidum polysaccharide on renal tubular endothelial-to-mesenchymal transition of diabetic nephropathy mice
ZHONG Tao1 ZHANG Guoxin1 ZHOU Diyi2 ZHU Tiane1▲
1.Department of Pharmaceutical Preparation Section, Huzhou Northern Zhejiang Mingzhou Hospital, Zhejiang Province, Huzhou 313000, China;
2.Department of Endocrinology, Zhejiang Integrated Traditional and Western Medicine Hospital, Zhejiang Province, Hangzhou 310000, China
Abstract：Objective To investigate the effect of ganoderma lucidum polysaccha-ride (GLP) on epithelial-to-mysenchymal transition (EMT) of renal tubular epithelial cells in diabetic nephropathy (DN) mice. Methods Forty C57BL/KsJ-db/db mice were randomly divided into 5 groups by weight. Normal group was fed with normal feed. Model group, GLP low (100 mg/kg), high (400 mg/kg) dose groups and Irbesartan group (50 mg/kg, Irbesartan) were given high-sugar dieat. Each group was given the drug by gavage daily for 8 weeks after successful modeling of DN mice. The changes of renal function, blood lipid and other physiological indexes were measured. The protein expression and mRNA level of E-cadherin, α-SMA, TGF-β1, Fibronectin, Vimentin, N-cadherin, TGF-β1, p-Smad2, p-Smad3, Smad2 and Smad3 were measured by Western Blot and Real-time PCR. Results Compared with the model group, the renal function and blood lipid metabolism in GLP low and high doses group were significantly decreased (P ＜ 0.05). Compared with the model group, the levels of E-cadherin protein and mRNA were increased in the GLP low and high doses group and the Irbesartan group, while the levels of α-SMA, Fibronectin, Vimentin, N-cadherin protein and mRNA were decreased (P ＜ 0.01). The expression levels of Smad2 and Smad3 were unchanged. Conclusion GLP inhibits EMT maybe via inhibition of TGF-β1/Smad signaling pathway.
2020, Vol. 17 
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