Study on the relationship between obesity and single nucleotide polymorphism of obesity gene in children
ZHENG Yi1,2 HE Qi2,3 LI Yubo4 CHEN Jialiang1,2▲ LI Xiaodong2,5
1.Department of Clinical Laboratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Hubei Province, Wuhan 430061, China;
2.Hubei Province Academy of Traditional Chinese Medicine, Hubei Province, Wuhan 430074, China;
3.Department of Dermatological, Hubei Provincial Hospital of Traditional Chinese Medicine, Hubei Province, Wuhan 430061, China;
4.Institute of Basic Theory for Chinese Medicine, China Academy of Chinese Medicine Science, Beijing 100700, China;
5.Deparment of Hepatology, Hubei Provincial Hospital of Traditional Chinese Medicine, Hubei Province, Wuhan 430061, China
Abstract：Objective To study the mutation of obesity gene in children in Central China, and to explore the relationship between obesity and single nucleotide polymorphism (SNP) of obesity gene in children. Methods From May 2017 to May 2018, a total of 100 children aged five to ten were selected from the Department of Obesity and Internal Medicine of Hubei Hospital of Traditional Chinese Medicine. According to body mass index, they were divided into control group (24 cases), overweight group (42 cases) and obesity group (34 cases). Three SNP loci of obesity gene (rs9939609, rs1421085, rs8057044) were detected by the improved multiple high temperature ligase assay (iMLDR) and the mutations of SNP locus in three groups were detected by sequencing. Results There were no significant differences in genotype distribution of rs9939609 and rs1421085 loci among three groups (P > 0.05). Genotype distribution of RS8057044 locus were compared among three groups, and the differences were statistically significant (P < 0.05). There was no significant difference in allele frequency of rs8057044 locus between overweight group and obesity group (P > 0.05). Conclusion Among the children aged five to ten, rs8075044 locus can be used as a potential detection site for adult obesity.
郑毅1,2 贺琪2,3 李玉波4 陈家良1,2▲ 李晓东2,5. 儿童肥胖与肥胖基因单核苷酸多态性的关系研究[J]. 中国医药导报, 2020, 17(23): 8-11,19.
ZHENG Yi1,2 HE Qi2,3 LI Yubo4 CHEN Jialiang1,2▲ LI Xiaodong2,5. Study on the relationship between obesity and single nucleotide polymorphism of obesity gene in children. 中国医药导报, 2020, 17(23): 8-11,19.
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2020, Vol. 17 
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