Effects of Sanjie Tongluo Decoction on the expression of matrix metalloproteinase 2, tissue inhibitor of metalloproteinase 2 in rats with ischemic nephropathy of advanced stage
WANG Rui1 CHEN Guimei1 LI Wei1 LIU Lanying1▲ LI Jiaoyan1 LI Genmao2 GE Dongyu2 WANG Yaoxian3
1.Department of Nephrology, the Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China;
2.Basic Research Center, Beijing University of Chinese Medicine, Beijing 100029, China;
3.Department of Nephrology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
Abstract：Objective To investigate the effects of Sanjie Tongluo Decoction on the expression of matrix metalloproteinase 2 (MMP-2), tssue inhibitor of metalloproteinase-2 (TIMP-2) of nephridial tissue in rats with ischemic nephropathy of advanced stage. Methods Male SD rats were randomly divided into sham operation group, model group (both groups were given normal saline 3 mL/d), drug group [Sanjie Tongluo Decoction 10.85 g/(kg·d)] by random number table method. The unilateral renal artery insufficiency ligation was used to establish animal model. In the 28th day of experiment, the levels of 24 hours urinary protein and creatinine of rats were detected. The rats were put to death and nephridial tissue was collected to take light microscope examination, the changes of renal morphology in all groups were observed. Immunohistochemistry was used to detect the expression of MMP-2 and TIMP-2 of nephridial tissue. Results Under the light microscope, the glomerulus and tubulointerstitium in the model group and drug group had damage. Compared with sham operation group, the scores of glomerulosclerotic index and tubulointerstitial lesion in the model group and drug group were all increased (P ＜ 0.01), which of drug group were lower than those of model group (P ＜ 0.01). Compared with model group, the expression of MMP-2 in drug group was increased (P ＜ 0.01), 24 hours urinary protein, creatinine and the expression of TIMP-2 were decreased (P ＜ 0.01), which of both groups were higher than those of sham operation group (P ＜ 0.01). Conclusion Sanjie Tongluo Decoction can prevent the lesions of ischemic nephropathy of advanced stage by up-regulating the expression of MMP2 and down-regulating the expression of TIMP-2.
王蕊1 陈桂梅1 李伟1 刘兰英1▲ 李娇艳1 李根茂2 葛东宇2 王耀献3. 散结通络方对缺血性肾病晚期大鼠MMP-2、TIMP-2表达的影响[J]. 中国医药导报, 2017, 14(29): 24-27.
WANG Rui1 CHEN Guimei1 LI Wei1 LIU Lanying1▲ LI Jiaoyan1 LI Genmao2 GE Dongyu2 WANG Yaoxian3. Effects of Sanjie Tongluo Decoction on the expression of matrix metalloproteinase 2, tissue inhibitor of metalloproteinase 2 in rats with ischemic nephropathy of advanced stage. 中国医药导报, 2017, 14(29): 24-27.
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