Abstract：Objective To compare the components before and after the inclusion of volatile oil from Amomum tsaoko, and the bacteriostatic test was carried out. Methods Gas chromatography-mass spectrometry (GC-MS) method was used: HP-5MS quartz capillary column (30 m×0.25 mm×0.25 mm); column temperature: the initial temperature was 40℃; the temperature was increased to 80℃ at 3℃/min, and then to 280℃ at 5℃/min; the column flow rate was 1.0 mL/min; inlet temperature was 250℃; sample injection volume was 1.0 mL; the split ratio was 10∶1; the carrier gas was high-purity helium gas. The standard filter paper method was used to conduct bacteriostatic tests on Candida albicans standard strains SC5314, Candida albicans clinical isolates of drug resistant strains 23#, Staphylococcus aureus and Escherichia coli. Results Sixty-eight compounds in the volatile oil from Amomum tsaoko and 64 inclusion compounds were isolated by GC-MS component analysis. There were 60 common compounds, accounting for 99.11% and 99.65% of the total volatile oil compounds and inclusion compounds, respectively. The bacteriostatic experiments showed that the volatile oil and its inclusion compound had good bacteriostatic effects on Candida albicans and drug-resistant bacteria, and were significantly better than Fluconazole. It also had good bacteriostatic effect on Staphylococcus aureus and Escherichia coli. Conclusion After ultrasonic inclusion, more than 99% of the volatile oil from Amomum tsaoko into beta-cyclodextrin, forming a stable inclusion compound. There is no significant difference in bacteriostatic effect of volatile oil from Amomum tsaoko on the four strains before and after inclusion.
刘娜 丁雄▲ 赵毅 苏刘艳 赵蓉羽. 草果挥发油及其包合物GC-MS成分分析及抑菌试验研究[J]. 中国医药导报, 2021, 18(1): 31-35.
LIU Na DING Xiong▲ ZHAO Yi SU Liuyan ZHAO Rongyu. GC-MS composition analysis and bacteriostatic test of volatile oil and its inclusion compound from Amomum tsaoko. 中国医药导报, 2021, 18(1): 31-35.
2021, Vol. 18 
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