Effects of miR-1203 and HOTAIR SNP rs7958904 on the growth of breast cancer cells
LIU Wenhui1 XIAO Jian2 SUN Weihua3 YANG Na3 LIU Boxuan3 GONG Jianping3
1.Department of Pharmacy, the Second Xiangya Hospital of Central South University, Hu'nan Province, Changsha 410008, China;
2.Department of Pharmacy, Xiangya Hospital of Central South University, Hu'nan Province, Changsha 410008, China;
3. Department of Pharmacy, Huaihua Second People's Hospital, Hu'nan Province, Huaihua 418000, China
Abstract：Objective To investigate the association between genetic variation of lncRNA HOTAIR SNP rs7958904 and miR-1203, as well as to explore their roles in the growth of breast cancer （BC） cells. Methods The wild type and mutant HOTAIR SNP rs-7958904 were both cloned into the Dual-Luciferase Reporter Vector and Over-expression vector, respectively. The Dual-Luciferase Reporter Vectors and miR-1203 mimics were co-transfected into the cells, and then the luciferase expression was detected. Furthermore, BC cells were transfected with the overexpression vector and/or miR-1203 mimics/inhibitors. And then, HOTAIR expression was detected by fluorescent quantitative PCR, cell proliferation was assessed by BrdU, cell apoptosis was tested by Annexin V-FITC/PI, and cell invasion was examined by Transwell Assay. Results The binding of miR-1203 and HOTAIR was enhanced by mutant HOTAIR; miR-1203 could down-regulate the expression of mutant HOTAIR （P < 0.01）, but such down regulation on wild-type HOTAIR was insignificant （P > 0.05）. The wild-type HOTAIR promoted the proliferation and invasion of BC cells significantly but inhibit their apoptosis （P < 0.01）; whereas such effect of the mutant HOTAIR declined obviously and could be blocked by miR-1203. miR-1203 had the opposite effect of HOTAIR; when the expression of miR-1203 was inhibited, the mutant HOTAIR exhibited a similar effect as wild-type HOTAIR. Conclusion The mutant HOTAIR rs7958904 can down-regulate the activity of HOTAIR, and such down regulation may probably associated with the increment of miR-1203 binding sites. This finding could provide a new direction for the future study in the function of lncRNA SNPs.
刘文辉1 肖坚2 孙维华3 杨娜3 刘伯轩3 龚建平3. miR-1203和HOTAIR rs7958904位点对乳腺癌细胞生长的影响[J]. 中国医药导报, 2018, 15(3): 8-12.
LIU Wenhui1 XIAO Jian2 SUN Weihua3 YANG Na3 LIU Boxuan3 GONG Jianping3. Effects of miR-1203 and HOTAIR SNP rs7958904 on the growth of breast cancer cells. 中国医药导报, 2018, 15(3): 8-12.
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2018, Vol. 15 
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