Discussion on mechanism of Zhuanyao Decoction in the treatment of degenerative lumbar spinal stenosis based on network pharmacology
LI Luguang1 ZHU Liguo1 GAO Jinghua1，2 GAO Chunyu1 WANG Baojian1 WEI Xu1 YIN Xunlu1 LI Hui1
1.The Second Department of Spine, Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China;
2.Beijing Key Laboratory of Orthopaedic Technology of Traditional Chinese Medicine, Beijing 100102, China
Abstract：Objective To explore the mechanism of Zhuanyao Decoction in the treatment of degenerative lumbar spinal stenosis (DLSS) based on network pharmacology. Methods The main active components and targets of Zhuanyao Decoction were obtained through TCMSP, BATMAN database and literature, and the “active ingredient-target” network of Zhuanyao Decoction was constructed by Cytoscape. The pathogenic target of DLSS was obtained by DisGeNET, GeneCards and OMIM disease database. The analysis of protein-protein interaction (PPI) was carried out on the String platform. The PPI network was constructed by Cytoscape and the main targets in the network were screened out according to the degree value. Metascape was used to analyze the biological process and pathway of Zhuanyao Decoction in the treatment of DLSS. Results Eighty-six main active components and corresponding 185 targets of Zhuanyao Decoction were screened, 1168 pathogenic targets of DLSS and 84 intersection targets of DLSS treated with Zhuanyao Decoction were screened. PPI showed that it was closely related to JUN, TNF, AKT1, MAPK1, and other targets. There were 1987 biological function items and 142 signal pathways obtained by GO biological function and KEGG pathway analysis. Conclusion Zhuanyao Decoction has the characteristics of multi-component, multi-target and multi-pathway in the treatment of DLSS.
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