Abstract：Objective To compare the results of Carbamazepine in serum of patients with epilepsy determined by enzyme-multiplied immunoassay technique (EMIT) and two-dimensional liquid phase chromatography(2D-HPLC). Methods The serum concentration of Carbamazepine in 67 epilepsy patients was determined by EMIT and 2D-HPLC, respectively. In 2D-HPLC method, two reversed phase liquid chromatography columns [ASTON-FAQ C8 (4.0 mm×100.0 mm, 3 μm, ANAX) and Diamonsil-C18 (4.6 mm×200.0 mm, 5 μm, Dikma) column] were used as the first and second separation dimensions, respectively. ASTON C18 (4.6 mm×10.0 mm, 3 μm, ANAX) column was used as a connector to transfer the eluent from the first dimensional column to the second column for further separation. The mobile phases of the two dimensions consisted of acetonitrile-H2O (55∶45, v/v) and acetonitrile-20 mmol/L ammonium acetate (20∶80, v/v, pH=7.0), respectively. The first dimensional column flow rate was 1.0 mL/min, the second dimensional column flow rate was 1.2 mL/min. The detection wavelength was 285 nm, and the column temperature was 40℃. Results There were no significant differences in the serum concentrations of Carbamazepine detected by EMIT and 2D-HPLC. A good correlation was obtained between the two methods. The regression equation was y(2D-HPLC)= 0.9876 × x(EMIT)-0.0313. Bland-Altman plots analysis showed the two methods were consistent. Conclusion Both EMIT and 2D-HPLC methods have good consistency and correlation in the quantification of Carbamazepine. The laboratory conditions and staffing should be taken into consideration when a method is chosen for monitoring the concentration of Carbamazepine.
柳航 王敏 金路 张晋萍. 酶放大免疫分析法与二维液相色谱法监测癫痫患者血清中卡马西平浓度的比较[J]. 中国医药导报, 2018, 15(21): 106-109.
LIU Hang WANG Min JIN Lu ZHANG Jinping. Comparison of EMIT and 2D-HPLC in the quantification of Carbamazepine in serum of patients with epilepsy. 中国医药导报, 2018, 15(21): 106-109.
2018, Vol. 15 
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