膳食纤维对大鼠脑缺血后PTEN/PI3K/Akt/NF-κB信号通路的影响

摘要
图/表
参考文献(27)
相关文章 (15)

全文: PDF (734 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的探讨添加膳食纤维对大鼠脑缺血后PTEN/PI3K/AKT/NF-κB信号通路的影响。方法随机选取24只大鼠颅内注射内皮素制作脑缺血模型，模型制作完成后4只大鼠死亡。剩余20只大鼠按照随机数字表法分为缺血组和缺血+膳食纤维组，每组10只；另随机取10只大鼠作为假手术组，大鼠颅内注射等量生理盐水作为对照。缺血组和假手术组给予正常饮食，缺血+膳食纤维组给予膳食纤维。检测缺血组与缺血+膳食纤维组大鼠的脑梗死体积，用Western blot检测各组大鼠梗死周边皮层p-PI3K、p-Akt、PTEN、NF-κB/p65的蛋白表达水平变化。结果缺血组与缺血+膳食纤维组脑梗死体积比较，差异无统计学意义（P ＞ 0.05）。缺血组的p-PI3K、p-Akt的蛋白表达水平低于假手术组，差异有统计学意义（P ＜ 0.05），缺血+膳食纤维组的p-PI3K、p-Akt的蛋白表达水平高于缺血组，差异有统计学意义（P ＜ 0.05）。缺血组的PTEN、NF-κB/p65的蛋白表达水平高于假手术组，差异有统计学意义（P ＜ 0.05），缺血+膳食纤维组的PTEN、NF-κB/p65的蛋白表达水平低于缺血组，差异有统计学意义（P ＜ 0.05）。结论脑梗死后通过添加膳食纤维上调PI3K/Akt信号，抑制NF-κB通路的激活。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	杨事达1 曲慧玲2

关键词 ：脑梗死, 膳食纤维, 肠道菌群, 炎症通路

Abstract：Objective To investigate the effects of dietary fiber supplementation on PTEN / PI3K / AKT / NF-κB signaling pathway after cerebral ischemia in rats. Methods Twenty-four rats were randomly selected to make cerebral ischemia model by intracranial injection of endothelin. Four rats died after the model was made. The remaining 20 rats were divided into ischemia group and ischemia + dietary fiber group according to random number table method, with ten rats in each group. In addition, ten rats were randomly selected as sham operation group and intracranial injection of equal amount of normal saline was used as control. Ischemia group and sham operation group were given normal diet, ischemia + dietary fiber group was given dietary fiber. The volume of cerebral infarction in ischemic group and ischemic + dietary fiber group was detected, and the protein expression levels of p-PI3K, p-Akt, PTEN and NF-κB/P65 in the peripheral cortex of rats in each group were detected by Western blot. Results There was no significant difference in cerebral infarction volume between ischemic group and ischemic + dietary fiber group (P ＞ 0.05). The protein expression levels of p-PI3K and p-Akt in the ischemic group were lower than those in the sham group, and the differences were statistically significant (P ＜ 0.05); the protein expression levels of p-PI3K and p-Akt in the ischemic + dietary fiber group were higher than those in the ischemic group, and the differences were statistically significant (P ＜ 0.05). The protein expression levels of PTEN and NF-κB/ P65 in the ischemic group were higher than those in the sham group (P ＜ 0.05), and the protein expression levels of PTEN and NF-κB/P65 in the ischemic + dietary fiber group were lower than those in the ischemic group, the differences were statistically significant (P ＜ 0.05). Conclusion After cerebral infarction, dietary fiber can up-regulate PI3K / Akt signal and inhibit NF-κB pathway activation.

Key words： Cerebral infarction Dietary fiber Intestinal flora Inflammatory pathways

基金资助:辽宁省自然科学基金项目（2020-MS-054）。

通讯作者: 曲慧玲（1986.7-），女，博士，副主任医师；研究方向：神经内科。

作者简介: 杨事达（1979.2-），男，硕士，主任技师；研究方向：临床免疫检验。

引用本文:

杨事达1 曲慧玲2. 膳食纤维对大鼠脑缺血后PTEN/PI3K/Akt/NF-κB信号通路的影响[J]. 中国医药导报, 2022, 19(7): 11-14.
YANG Shida1 QU Huiling2. Effects of dietary fiber on PTEN / PI3K / Akt / NF-κB signaling pathway after cerebral ischemia in rats#br#. 中国医药导报, 2022, 19(7): 11-14.

链接本文:

https://www.yiyaodaobao.com.cn/CN/ 或 https://www.yiyaodaobao.com.cn/CN/Y2022/V19/I7/11

[1] Ilaria D，Lisa G，Filippo U，et al. Protective Effect of Adenosine A 2B Receptor Agonist，BAY60-6583，Against Transient Focal Brain Ischemia in Rat [J]. Front Pharmacol，2021，11：588757.
[2] Lei L，Man W，Jikuai C，et al. Preventive Effects of Bacillus licheniformis on Heat Stroke in Rats by Sustaining Intestinal Barrier Function and Modulating Gut Microbiota [J]. Front Microbiol，2021，12：630841.
[3] Austin V，Ku JM，Miller AA，et al. Ischaemic stroke in mice induces lung inflammation but not acute lung injury [J]. Sci Rep，2019，9（1）：3622.
[4] Feng Z，Mengting Z，Qian W，et al. Protective Effect of Tong-Qiao-Huo-Xue Decoction on Inflammatory Injury Caused by Intestinal Microbial Disorders in Stroke Rats [J]. Biol Pharm Bull，2020，43（5）：788-800.
[5] Becker AM，Meyers E，Sloan A，et al. An automated task for the training and assessment of distal forelimb function in a mouse model of ischemic stroke [J]. J Neurosci Methods，2016，258：16-23.
[6] Singh V，Roth S，Llovera G，et al. Microbiota dysbiosis controls the neuroinflammatory response after stroke [J]. J Neurosci，2016，36（28）：7428-7440.
[7] Lee J，d’Aigle J，Atadja L，et al. Gut Microbiota-Derived Short-Chain Fatty Acids Promote Poststroke Recovery in Aged Mice [J]. Circ Res，2020，127（4）：453-465.
[8] Sadler R，Cramer JV，Heindl S，et al. Short-Chain Fatty Acids Improve Poststroke Recovery via Immunological Mechanisms [J]. J Neurosci，2020，40（5）：1162-1173.
[9] Chen R，Xu Y，Wu P，et al. Transplantation of fecal microbiota rich in short chain fatty acids and butyric acid treat cerebral ischemic stroke by regulating gut microbiota [J]. Pharmacol Res，2019，148：104403.
[10] Haghikia A，J?觟rg S，Duscha A，et al. Dietary fatty acids directly impact central nervous system autoimmunity via the small intestine [J]. Immunity，2015，43（4）：817-829.
[11] Soleman S，Yip P，Leasure JL，et al. Sustained sensorimotor impairments after endothelin-1 induced focal cerebral ischemia（stroke）in aged rats [J]. Exp Neurol, 2010，222（1）：13-24.
[12] Biernaskie J, Corbett D. Enriched rehabilitative training promotes improved forelimb motor function and enhanced dendritic growth after focal ischemic injury [J]. J Neurosci，2001，21（14）：5272-5280.
[13] 张茹，郭荷娜，吴海琴，等.葛根素对大鼠局灶性脑缺血后钙超载的保护作用[J].南方医科大学学报，2010，30（6）：1268-1271.
[14] 李海涛，曲波，杨事达.添加膳食纤维对大鼠脑缺血后炎性反应的影响[J].中国医药导报，2020，17（20）：10-12，198.
[15] Patnala R，Arumugam TV，Gupta N，et al. HDAC Inhibitor Sodium Butyrate-Mediated Epigenetic Regulation Enhances Neuroprotective Function of Microglia During Ischemic Stroke [J]. Mol Neurobiol，2017，54（8）：6391-6411.
[16] Colombo AV，Sadler RK，Llovera G，et al. Microbiota-derived short chain fatty acids modulate microglia and promote Aβ plaque deposition [J]. Elife，2021，10：e59826.
[17] Zhang P，Zhang X，Huang Y，et al. Atorvastatin alleviates microglia-mediated neuroinflammation via modulating the microbial composition and the intestinal barrier function in ischemic stroke mice [J]. Free Radic Biol Med，2021， 162：104-117.
[18] Salter MW，Stevens B. Microglia emerge as central players in brain disease [J]. Nat Med，2017，23（9）：1018-1027.
[19] Tu XK，Yang WZ，Shi SS，et al. Spatio-temporal distribution of inflammatory reaction and expression of TLR2/4 signaling pathway in rat brain following permanent focal cerebral ischemia [J]. Neurochem Res，2010，35（8）：1147-1155.
[20] Kerr F，Rickle A，Nayeem N，et al. PTEN，a negative regulator of PI3 kinase signalling，alters tau phosphorylation in cells by mechanisms independent of GSK-3 [J]. FEBS Lett，2006，580（13）：3121-3128.
[21] Xing Y，Wang MM，Feng YS，et al. Possible involvement of PTEN signaling pathway in the anti-apoptotic effect of electroacupuncture following ischemic stroke in rats [J]. Cell Mol Neurobiol，2018，38（8）：1453-1463.
[22] Miao SY，Miao SM，Cui RT，et al. SETD5-AS1 stimulates neurondeath in stroke via promoting PTEN expression [J]. Eur Rev Med Pharmacol Sci，2018，22（18）：6035-6041.
[23] Zhao D，Chen J，Zhang Y，et al. Glycine confers neuroprotection through PTEN/AKT signal pathway in experimental intracerebral hemorrhage [J]. Biochem Biophys Res Commun，2018，501（1）：85-91.
[24] Zheng T，Shi Y，Zhang J，et al. MiR-130a exerts neuroprotective effects against ischemic stroke through PTEN/PI3K/AKT pathway [J]. Biomed Pharmacother，2019， 117：109117.
[25] Peng H，Yang H，Xiang X，et al. MicroRNA-221 participates in cerebral ischemic stroke by modulating endothelial cell function by regulating the PTEN/PI3K/AKT pathway [J]. Exp Ther Med，2020，19（1）：443-450.
[26] Dalile B，Van Oudenhove L，Vervliet B，et al. The role of short-chain fatty acids in microbiota-gut-brain communication [J]. Nat Rev Gastroenterol Hepatol，2019，16 （8）：461-478.
[27] Wang Y，Dilidaxi D，Wu Y，et al. Composite probiotics alleviate type 2 diabetes by regulating intestinal microbiota and inducing GLP-1 secretion in db/db mice [J]. Biomed Pharmacother，2020，125：109914.