Protective effect of Shuxuetong Injection on acute pulmonary embolism rats and its effect on cell apoptosis#br#
ZHANG Shengbi1 LIU Yanling2 XU Liying3
1.Department of Pharmacy, Wenzhou Hospital of Traditional Chinese Medicine, Zhejiang Province, Wenzhou 325000, China;
2.Pharmacy of Traditional Chinese Medicine, Jinhua Hospital of Traditional Chinese Medicine, Zhejiang Province, Jinhua 321017, China;
3.Department of Respiratory Medicine, Zhejiang Hospital of Traditional Chinese Medicine, Zhejiang Province, Hangzhou 310000, China
Abstract：Objective To study the protective effect of Shuxuetong Injection on acute pulmonary embolism (APE) and its effect on cell apoptosis. Methods Seventy-five SPF male SD rats aged seven weeks and weighing (250±20) g were divided into sham operation group, model group, Shuxuetong low-dose, medium-dose and high-dose groups according to random number table method, with 15 rats in each group. Except for the sham operation group, the APE model was prepared by autologous thrombosis transfusion, and the sham operation group was injected with the same amount of normal saline. Two hours before modeling, Shuxuetong dose groups were subcutaneously injected with 0.25, 0.5 and 1 ml/kg Shuxuetong Injection, and sham operation group and model group were subcutaneously injected with Dimethyl sulfoxide. The rats were anesthetized four hours after operation, the mean pulmonary artery pressure (mPAP) and right ventricular pressure (RVP) were detected, blood gas analysis, lung wet-dry ratio, oxidative stress, and pathological indicators of lung were detected, cell apoptosis was detected by TUNEL staining, and protein expression levels of Bcl-2, Bax, and Caspase-3 were detected by Western blot. Results Compared with sham operation group, the levels of mPAP and RVP in model group were increased (P ＜ 0.01). Compared with model group, mPAP level in Shuxuetong medium-dose and high-dose groups was decreased, while RVP level in Shuxuetong high-dose group was decreased (P ＜ 0.05). Compared with Shuxuetong low-dose group, the mPAP level in Shuxuetong medium-dose and high-dose groups was decreased (P ＜ 0.05). Compared with sham operation group, arterial partial pressure of oxygen (PaO2) in model group was decreased (P ＜ 0.01); compared with model group, the level of PaO2 in Shuxuetong high-dose group was increased (P ＜ 0.01). There was no significant difference in arterial blood partial pressure of carbon dioxide among all groups (P > 0.05). The lung tissue of the model group was seriously injured, while the lung tissue of Shuxuetong high-dose, medium-dose and low-dose groups showed some improvement, and the improvement was more obvious in Shuxuetong high-dose group. Compared with sham operation group, lung wet-dry ratio in model group was increased (P ＜ 0.01). Compared with model group and Shuxuetong low-dose group, the lung wet-dry weight ratio in Shuxuetong high-dose group was decreased (P ＜ 0.05). Compared with sham operation group, the activity levels of superoxide dismutase (SOD) and glutathione (GSH) in model group were decreased, while the activity level of myeloperoxidase (MPO) was increased (P ＜ 0.01). Compared with model group, SOD activity level of Shuxuetong medium-dose and high-dose groups was increased, MPO activity level was decreased, GSH activity level of Shuxuetong high-dose group was increased (P ＜ 0.05 or P ＜ 0.01). Compared with Shuxuetong low-dose and medium-dose groups, MPO activity in Shuxuetong high-dose group was decreased (P ＜ 0.01), and compared with Shuxuetong low-dose group, GSH activity in Shuxuetong high-dose group was increased (P ＜ 0.05). There were a lot of apoptosis in model group, the apoptosis of Shuxuetong low-dose group was alleviated, and the apoptosis of Shuxuetong medium-dose and high-dose groups was improved significantly. Compared with sham operation group, the expression levels of Bax and Caspase-3 protein in the lung tissues of model group were increased, while the expression level of Bcl-2 protein was decreased (P ＜ 0.01). Compared with model group, Bax protein expression level in the lung tissues of Shuxuetong medium-dose and high-dose groups was decreased, Bcl-2 protein expression level was increased, and Caspase-3 protein expression level in the lung tissues of Shuxuetong high-dose group was decreased (P ＜ 0.05). Compared with Shuxuetong low-dose group , the Bax and Caspase-3 protein expression levels in Shuxuetong high-dose group were decreased, while the Bcl-2 protein expression level was increased (P ＜ 0.05 or P ＜ 0.01). Compared with Shuxuetong medium-dose group, the expression level of Bax protein in the lung tissue of Shuxuetong high-dose group was decreased (P ＜ 0.05). Conclusion Shuxuetong Injection can effectively improve pulmonary embolism injury and inhibit apoptosis in rats.
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