Effects of vitamin D intervention on balance regulation of peripheral blood T lymphocytes and intestinal flora in young rats with bronchial asthma
CAI Zixiu1 ZHOU Guohua1 TIAN Jun2▲
1.Department of Pediatrics, the First People’s Hospital of Xiaoshan District, Hangzhou City, Zhejiang Province, Hangzhou 311200, China;
2.Department of Pediatrics, the Third People’s Hospital of Xiaoshan District, Hangzhou City, Zhejiang Province, Hangzhou 311256, China
Abstract：Objective To investigate the effects of vitamin D (VitD) intervention on the balance regulation of peripheral blood T lymphocytes and intestinal flora in young rats with bronchial asthma (hereinafter referred to as “asthma”). Methods Thirty SPF wild-type BALB/c female mice (4.09 - 6.13 g) aged seven days were selected as the study objects, and they were divided into asthma model group (15 rats) and VitD intervention group (15 rats) by random number table method, another ten rats were selected as healthy control group. On the first and seventh day of the experiment, asthma model group and VitD intervention group were injected intraperitoneally with 10% ovalbumin (OVA) sensitizing solution 200 μl, while healthy control group was injected with the same amount of normal saline. On the 18th day, VitD intervention group was given VitD drops (35 μg/kg, per week, one every two days) for two weeks, and healthy control group and asthma model group were given the same amount of phosphate buffer. On the 19th day, asthma model group and VitD intervention group were given atomized inhalation of 3%OVA solution 10 ml for 20 min, while healthy control group was given atomized inhalation of normal saline, once a day for six days. After the experiment, the hematoxylin-eosin staining results of lung tissue sections in three groups were observed. Serum Th1/Th2 and Th17/ regulatory T cell (Treg) ratios were measured and calculated by flow cytometry. The bacterial community in feces of the three groups was analyzed by 16S rDNA sequencing method. Results In the healthy control group, the structure of the bronchial wall and alveolar wall of the lung tissue were complete, there was no exudation in the alveolar cavity, the alveolar epithelium was orderly, and the alveolar wall thickness was normal. In asthmatic model group, the structure of alveolar wall was damaged, alveolar epithelial cells were destroyed, mucosal hyperemia and edema were observed, and perivascular inflammatory cells were infiltrated. The inflammatory reaction of lung tissue in VitD intervention group was less. The Shannon index curve of microbial species in the three groups was flat, and there was no statistical significance in Simpson index among the three groups (P＞0.05). PCoA diagram analysis showed that the distance between the asthma model group and the healthy control group was large, and the distance between the VitD intervention group and the asthma model group was close (P＜0.05). The three groups of intestinal flora involved 15 divisions and 23 genera. Th1/Th2 ratio and the abundance of Actinobacteriota, Helicobacter, and Colidextribacter in asthma model group were lower than those in healthy control group, Th17/Treg ratio and the abundance of Proteobacteria, Ligilactobacillus, and Bacteroides of asthma model group were higher than those of healthy control group (P＜0.05). Th1/Th2 ratio and the abundance of Actinobacteriota, Proteobacteria, Helicobacter, and Colidextribacter in VitD intervention group were higher than those in asthma model group, The Th17/Treg ratio and the abundance of Ligilactobacillus, Bacteroides in VitD intervention group were lower than those of the asthma model group (P＜0.05), which were consistent with the result of heatmap analysis based on genera level. Conclusion VitD intervention can regulate the balance of peripheral blood T lymphocytes and change the intestinal flora of young mice with asthma, which may be a new entry point for the pathogenesis of this disease.
蔡姿秀1 周国华1 田军2▲. 维生素D干预对支气管哮喘幼鼠外周血T淋巴细胞平衡调节及肠道菌群的影响[J]. 中国医药导报, 2023, 20(16): 21-25,36.
CAI Zixiu1 ZHOU Guohua1 TIAN Jun2▲. Effects of vitamin D intervention on balance regulation of peripheral blood T lymphocytes and intestinal flora in young rats with bronchial asthma. 中国医药导报, 2023, 20(16): 21-25,36.
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