131例强直性脊柱炎患者Treg细胞与淋巴细胞亚群分析

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摘要目的观察强直性脊柱炎（AS）患者外周血Treg细胞与淋巴细胞各亚群的变化及HLA-B27的表达，探讨各自在AS患者发展中的作用。方法应用流式细胞学方法，选取2013年2月～2016年12月于无锡市第二人民医院骨科和风湿科就诊的AS患者131例为AS组，选取健康体检者127例为正常对照组，进行外周血Treg细胞、淋巴细胞亚群、HLA-B27的检测，分析Treg细胞、淋巴细胞亚群、HLA-B27与AS患者的关系。结果 131例AS组中HLA-B27检测阳性为123例（93.89%），正常对照组中HLA-B27检测阳性为2例（1.57%），差异有统计学意义（P < 0.05）。AS组患者CD3+CD4+、CD4/CD8、CD3-CD19+阳性率明显高于正常对照组（P < 0.05），CD3+CD8+、CD16+/CD56+阳性率低于正常对照组（P < 0.05）。AS组患者CD4+CD25high、CD4+CD25highCD127low阳性率均低于正常对照组，差异有统计学意义（P < 0.05）。两组CD3+、CD3+CD4+阳性率比较差异无统计学意义（P ＞ 0.05）。结论 HLA-B27与AS有强相关性。外周血中淋巴细胞各亚群组分在AS与正常对照组中存在明显差异。同时外周血中Treg细胞在AS患者中明显降低。以上发现提示细胞免疫和体液免疫可能在AS中发挥重要作用。

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	魏云玉1 韩志君1▲ 黄红宇1 杜伟2 任天丽3 高明珠1

关键词 ：强直性脊柱炎, Treg细胞, 淋巴细胞亚群, HLA-B27

Abstract：Objective To analyze the changes of regulatory T cells (Treg cells) and the subsets of lymphocytes as well as the HLA-B27 expression in peripheral blood of ankylosing spondylitis (AS) patients, and then explore their roles in the development of AS. Methods Flow cytometry was applied. From February 2013 to December 2016, Department of Orthopedic and Rheumatology in Wuxi Second People's Hospital, 131 cases of AS patients were selected as AS group, 127 healthy subjects were selected as normal control group. The peripheral blood Treg cells, lymphocyte subsets and HLA-B27 were detected. The relationship of Treg cells, lymphocyte subsets, HLA-B27 and AS patients were analyzed. Results 131 cases of AS group, HLA-B27 positive for 123 cases (93.89%), the positive rate of HLA-B27 in normal control group was 2 cases (1.57%), the difference was statistically significant (P < 0.05). The positive rate of CD3+CD4+, CD4/CD8 and CD3-CD19+ in AS group were significantly higher than those in normal control group (P < 0.05). The positive rate of CD3+CD8+, CD16+/CD56+ in AS group were lower than those in normal control group (P < 0.05). The positive rate of CD4+CD25high and CD4+CD25highCD127low in AS group were lower than those in normal control group, the differences were statistically significant (P < 0.05). There was no significant difference in the positive rate of CD3+ and CD3+CD4+ between two groups (P > 0.05). Conclusion HLA-B27 has a strong correlation with AS. There are significant differences in the subgroups of lymphocytes in peripheral blood between AS and normal control group. At the same time, peripheral blood Treg cells in AS patients are significantly reduced. The above findings suggest that cellular immunity and humoral immunity may play an important role in AS.

Key words： Ankylosing spondylitis Regulatory T cells Lymphocyte subsets HLA-B27

基金资助:国家自然科学基金资助项目（81301503）；
南京医科大学科技发展基金面上项目（2014NJMU062）。

通讯作者: ▲通讯作者

作者简介: 魏云玉，男，副主任技师，主要从事自身免疫病的研究。

引用本文:

魏云玉1 韩志君1▲ 黄红宇1 杜伟2 任天丽3 高明珠1. 131例强直性脊柱炎患者Treg细胞与淋巴细胞亚群分析[J]. 中国医药导报, 2017, 14(28): 46-48.
WEI Yunyu1 HAN Zhijun1▲ HUANG Hongyu1 DU Wei2 REN Tianli3 GAO Mingzhu1. Analysis of Treg cells and lymphocyte subgroup in 131 patients with ankylosing spondylitis. 中国医药导报, 2017, 14(28): 46-48.

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http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2017/V14/I28/46

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