Study on the fingerprint and antioxidant activity of Xwak Granules
CHEN Li1,2 LIU Liu1 MA Qingling1 Xirali Tursun3 WU Tao1 XIN Xuelei1 Haji Akber Aisa1
1.Key Lab of Chemistry of Plant Resources in Arid Regions, the Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Xinjiang Uygur Autonomous Region, Urumqi 830011, China;
2.Graduate School, University of Chinese Academy of Sciences, Beijing 100049, China;
3.Key Laboratory of Uighur Medical Prescription and Pharmacology, Uighur Medical Research Institute, Xinjiang Uygur Autonomous Region, Urumqi 830049, China
Abstract：Objective To establish the high performance liquid chromatography and thin layer chromatography fingerprints of Xwak Granules, simultaneously determine the contents of five index components, and preliminarily investriage the antioxidant activity of Xwak Granules. Methods High performance liquid chromatography was performed on InertSustain C18 column. The mobile phase was acetonitrile-0.2% phosphoric acid, and the column temperature was 30℃. The segmented detection wavelengths were 245, 260, 300, 320, 350 and 360 nm, the flow rate was 1 mL/min, and the injection volume was 10 μL. The similarity analysis was carried out by the “similarity evaluation system for traditional Chinese medicine chromatographic fingerprint (2004A edition)”. A method for simultaneous determination of five characteristic compounds was established. The antioxidant activity was investigated by thin layer chromatography-bioautoradiography method. Results The similarity of the fingerprints of ten different batches of Xwak Granules was higher than 0.811, 36 common peaks were identified. Five characteristic peaks were identified as gallic acid, 3,5-O-dicaffeoylquinic acid, isoquercitrin, 4,5-O-dicaffeoylquinic acid and rupestonic acid. The linear coefficients of Xwak Granules content determination method were more than 0.9981. The relative standard deviations of precision, reproducibility, stability and recovery of characteristic peaks were all lower than 3%. Meanwhile, the contents of these five index components were determined. Xwak Granules had certain scavenging effects on 2,2’-amino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH). Conclusion The established fingerprint method is accurate, efficient and reproducible. It can be used for the quality control of Xwak Granules and the content of five components can be determined simultaneously. Xwak Granules contain many components which can scavenging ABTS and DPPH free radicals and have certain antioxidant activity.
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