Expression and clinical significance of long non-coding RNA plasmacytoma variant translocation 1 and microRNA-195-5p in thyroid carcinoma tissue
LI Xiaoling1 SHAO Xin1 GUO Ling2 YANG Yali3
1.Department of Pathology, Changzhou Hospital of Traditional Chinese Medicine, Jiangsu Province, Changzhou 213000, China;
2.Department of Pathology, Qidong Hospital Affiliated to Nantong University, Jiangsu Province, Qidong 226200, China;
3.Department of Pathology, the Second People’s Hospital of Yunnan Province, Yunnan Province, Kunming 650021, China
Abstract：Objective To investigate the expression and clinical significance of long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) and microRNA (miRNA)-195-5p in thyroid carcinoma tissue. Methods The expression of lncRNA PVT1 and miR-195-5p in 82 cases of thyroid cancer tissues, adjacent normal tissues and 82 cases of benign thyroid tumor tissues treated in Changzhou of Hospital Traditional Chinese Medicine from April 2017 to January 2020 were detected by fluorescence real-time quantitative PCR (qRT-PCR), the relationship between the two indexes and clinicopathological parameters of patients with thyroid cancer and the correlation between the two inedxes was analyzed. Results The relative expression level of lncRNA PVT1 in thyroid cancer tissues was higher than that in benign thyroid tumor tissues and paracancerous normal tissues, while the relative expression level of miR-195-5p was lower than that in benign thyroid tumor tissues and paracancerous normal tissues (all P ＜ 0.05). In thyroid cancer, the relative expression level of lncRNA PVT1 with lymph node metastasis and TNM stage Ⅲ-Ⅳ stage was higher than that without lymph node metastasis and Ⅰ-Ⅱ stage. The relative expression level of miR-195-5p in lymph node metastasis and TNM stage Ⅲ-Ⅳ stage was lower than that in the non-lymph node metastasis and Ⅰ-Ⅱ stage (all P ＜ 0.05).The expression of lncRNA PVT1 in thyroid cancer tissues was negatively correlated with the expression of miR-195-5p (r = -0.731, P < 0.01). Conclusion The increased lncRNA PVT1 and decreased miR-195-5p in thyroid carcinoma tissues are related to lymph node metastasis and TNM stage, which have potential application value for assessment of patients’ condition and determining therapeutic targets.
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