New progress in the study of differentiation of bone marrow mesenchymal stem cells into cardiomyocytes induced by Chinese medicine monomer
DU Nannan1 WANG Yinfang2 ZHANG Peng2 LIU Zongjun1
1.Department of Cardiovascular Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China;
2.Central Laboratory, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China
Abstract:For a long time, cardiovascular diseases have threatened human life and health, and myocardial infarction is one of the most lethal diseases. Myocardial injury and apoptosis after myocardial infarction largely affect the quality of life of patients. Cardiomyocyte regeneration is the key to solve this problem. Based on the physiological characteristics of cardiomyocytes and bone marrow mesenchymal stem cells, this paper elaborates the signaling pathways and molecular mechanisms related to the directional differentiation of bone marrow mesenchymal stem cells into cardiomyocytes, and summarizes the regulatory mechanism and functional characteristics of traditional Chinese medicine in inducing differentiation, in order to provide reference for the prevention and treatment of cardiovascular diseases by traditional Chinese medicine.
杜楠楠1 汪引芳2 张鹏2 刘宗军1. 中药单体诱导骨髓间充质干细胞向心肌细胞分化的研究新进展[J]. 中国医药导报, 2019, 16(23): 30-33.
DU Nannan1 WANG Yinfang2 ZHANG Peng2 LIU Zongjun1. New progress in the study of differentiation of bone marrow mesenchymal stem cells into cardiomyocytes induced by Chinese medicine monomer. 中国医药导报, 2019, 16(23): 30-33.
[1] Fukuhara S,Tomita S,Yamashiro S,et al. Direct cell-cell interaction of cardiomyocytes is key for bone marrow stromal cells to go into cardiac lineage in vitro [J]. J Thorac Cardiov Sur,2003,125(6):1470-1480.
[2] Melo LG,Pachori AS,Kong D,et al. Molecular and cell-based therapies for protection,rescue,and repair of ischemic myocardium - Reasons for cautious optimism [J]. Circulation,2004,109(20):2386-2393.
[3] Soltani L,Rahmani HR,Joupari MD,et al. Ovine fetal mesenchymal stem cell differentiation to cardiomyocytes,effects of co-culture,role of small molecules;reversine and 5-azacytidine [J]. Cell Biochem Funct,2016,34(4):250-261.
[4] Xing Y,Lv A,Wang L,et al. The combination of angiotensin Ⅱ and 5-azacytidine promotes cardiomyocyte differentiation of rat bone marrow mesenchymal stem cells [J]. Mol Cell Biochem,2012,360(1/2):279-287.
[5] Ling SK,Wang R,Dai ZQ,et al. Pretreatment of rat bone marrow mesenchymal stem cells with a combination of hypergravity and 5-azacytidine enhances therapeutic efficacy for myocardial infarction [J]. Biotechnol Prog,2011,27(2):473-482.
[6] Hafez P,Jose S,Chowdhury SR,et al. Cardiomyogenic differentiation of human sternal bone marrow mesenchymal stem cells using a combination of basic fibroblast growth factor and hydrocortisone [J]. Cell Biol Int,2016,40(1):55-64.
[7] Zhang GW,Liu XC,Li-Ling J,et al. Mechanisms of the protective effects of BMSCs promoted by TMDR with heparinized bFGF-incorporated stent in pig model of acute myocardial ischemia [J]. J Cell Mol Med,2011,15(5):1075-1086.
[8] Ybarra N,Vincent P,Smith LC,et al. Oxytocin improves the expression of cardiac specific markers in porcine bone marrow stem cells differentiation [J]. Res Vet Sci,2015, 98:42-50.
[9] Wang Y,Zhang G,Wang H,et al. Preinduction with bone morphogenetic protein-2 enhances cardiomyogenic differentiation of c-kit + mesenchymal stem cells and repair of infarcted myocardium [J]. Int J Cardiol,2018,265:173-180.
[10] 苗忠澄,高航.BMP-2预诱导骨髓间充质干细胞移植治疗大鼠再灌注后心肌梗死的实验研究[J].解放军医学杂志,2014,39(8):601-608.
[11] Shen X,Pan B,Zhou H,et al. Differentiation of mesenchymal stem cells into cardiomyocytes is regulated by miRNA-1-2 via WNT signaling pathway [J]. J Biomed Sci,2017,24(1):29.
[12] Ren M,Wang T,Huang L,et al. Role of VR1 in the differentiation of bone marrow-derived mesenchymal stem cells into cardiomyocytes associated with Wnt / beta-catenin signaling [J]. Cardiovasc Ther,2016,34(6):482-488.
[13] Lobanok ES,Kvacheva ZB,Pinchuk SV,et al. The influence of fibroblast growth factor (FGF2) on cardiomyocytes differentiation of mesenchymal stem cells of bone marrow ex vivo [J]. Biofizika,2014,59(2):360-368.
[14] Ding R,Jiang X,Ha Y,et al. Activation of Notch1 signalling promotes multi-lineage differentiation of c-Kit(POS)/NKX2.5(POS) bone marrow stem cells:implication in stem cell translational medicine [J]. Stem Cell Res Ther,2015,6:91.
[15] Yu Z,Zou Y,Fan J,et al. Notch1 is associated with the differentiation of human bone marrowderived mesenchymal stem cells to cardiomyocytes [J]. Mol Med Rep,2016, 14(6):5065-5071.
[16] 刘昕超.猪骨髓间充质干细胞体外向心肌样细胞分化过程中Notch4下调[D].北京:北京协和医学院,2009.
[17] Wang C,Du H,Hou J,et al. Chaihulonggumulitang Shows Psycho-cardiology Therapeutic Effects on Acute Myocardial Infarction by Enhancing Bone Marrow Mesenchymal Stem Cells Mobilization [J]. Sci Rep-Uk,2018,8(1):3724.
[18] 孙敬和,冼绍祥,黄习文,等.中医药干预骨髓间充质干细胞体外定向诱导分化为心肌细胞的研究进展[J].中药新药与临床药理,2012,23(1):115-118.
[19] 王怡,高秀梅,邢永发,等.丹参酚酸B、丹参酮治疗心血管疾病的药理学研究进展[J].上海中医药杂志,2010, 44(7):82-87.
[20] Lv Y,Liu B,Liu Y,et al. TGF-beta1 combined with Sal-B promotes cardiomyocyte differentiation of rat mesenchymal stem cells [J]. Exp Ther Med,2018,15(6):5359-5364.
[21] Lv Y,Gao CW,Liu B,et al. BMP-2 combined with salvianolic acid B promotes cardiomyocyte differentiation of rat bone marrow mesenchymal stem cells [J]. Kaohsiung J Med Sci,2017,33(10):477-485.
[22] Chen J,Wei J,Huang Y,et al. Danhong Injection Enhances the Therapeutic Efficacy of Mesenchymal Stem Cells in Myocardial Infarction by Promoting Angiogenesis [J]. Front Physiol,2018,9:991.
[23] 张宝霞,张金生,张阳阳,等.三七总皂苷、红景天苷、黄芪有效组分对心肌梗死大鼠模型骨髓间充质干细胞动员作用研究[J].中国实验方剂学杂志,2016,22(8):137-142.
[24] 宋雪云,龙云,谭超,等.黄芪甲苷联合丹参酮Ⅱa诱导骨髓间充质干细胞向心肌样细胞分化[J].中国组织工程研究,2013,17(36):6515-6520.
[25] 孙敬和.心肌样微环境下黄芪甲苷联合5-氮胞苷诱导MSCs分化为心肌样细胞[D].广州:广州中医药大学,2012.
[26] 王乐,田立,郑明奇,等.人参皂苷Rh2干预催产素诱导骨髓间充质干细胞向心肌细胞的转化[J].中国组织工程研究,2015,19(23):3633-3638.
[27] 汪朝晖,冼绍祥,杨忠奇,等.人参总皂甙诱导骨髓间充质干细胞分化为心肌样细胞的实验研究[J].广州中医药大学学报,2006,23(2):100-103.
[28] 王俊东,崔勇,王建国,等.人参皂苷Rh3预处理对大鼠心肌缺血再灌注损伤的保护作用研究[J].中华中医药学刊,2017,35(11):2783-2786.
[29] 孔晓丹,曲鹏,王彦,等.三七皂甙诱导骨髓基质细胞分化为心肌样细胞的实验研究[J].中国心血管病研究杂志,2005,3(7):528-530.
[30] 杨忠奇,冼绍祥,汪朝晖,等.三七总皂苷对骨髓间充质干细胞分化为心肌样细胞的作用[J].中药新药与临床药理,2006,17(4):239-242.
[31] 谢玲玲.淫羊藿苷诱导骨髓间充质干细胞分化心肌样细胞的实验研究[D].广州:广州中医药大学,2008.
[32] 孙晓冬,董韬,于宏伟,等.淫羊藿苷体外诱导小鼠胚胎干细胞分化为心肌细胞[J].解剖科学进展,2011,17(2):124-127.
[33] 刘韶英.淫羊藿苷对骨髓间充质干细胞增殖及向心肌细胞分化的影响[D].北京:北京中医药大学,2008.
[34] 戴儒丽.川芎嗪对骨髓间质干细胞增殖、SDF-1mRNA表达及向心肌样细胞分化的实验研究[D].广州:广州中医药大学,2010.
[35] 谢娟.益气活血中药对骨髓间充质干细胞向缺血心肌迁移的影响及其机制研究[D].西安:第四军医大学,2012.
[36] 陈梅,王立新.中药联合干细胞疗法治疗缺血性脑卒中的研究进展[J].中国实验方剂学杂志,2017,23(19):218-226.
[37] 林惠.丹参注射液干预脑梗死大鼠SVZ干细胞增殖作用的研究[D].长沙:湖南中医药大学,2014.
[38] 李荣,王向培,王创畅,等.参附注射液预处理对5-氟胞苷体外诱导兔骨髓间充质干细胞转化为心肌样细胞的实验研究[J].中药新药与临床药理,2015,26(6):765-770.
[39] 张爱国,蔡建平,谭湘陵,等.五种中药对体外培养的大鼠骨髓间充质干细胞增殖活性的作用[J].辽宁中医杂志,2012,39(1):158-161.
[40] 王新华,王士雯,李泱,等.地黄低聚糖诱导骨髓间充质干细胞向心肌样细胞分化的实验研究[J].解放军医学杂志,2009,34(4):412-414.
[41] 雷娄芳.柴胡三参胶囊含药血清对P38MAPK通路介导BMSCs向心肌细胞分化的干预研究[D].长沙:湖南中医药大学,2017.
[42] 武重阳,孙兰军,赵英强,等.复方丹参滴丸含药血清诱导大鼠骨髓间充质干细胞分化为心肌样细胞[J].中国老年学杂志,2010,30(16):2328-2330.
[43] 李伟娟,谢保平,石丽颖,等.从ERα/RANK通路探讨淫羊藿苷抑制破骨细胞分化作用[J].中国实验方剂学杂志,2017,23(7):121-126.