1.Department of Pediatrics, the 988th Hospital of Logistic Support Forces of PLA, He′nan Province, Zhengzhou 450042, China;
2.Department of Pharmacy, the 988th Hospital of Logistic Support Forces of PLA, He′nan Province, Zhengzhou 450042, China;
3.Department of Pathology, the 988th Hospital of Logistic Support Forces of PLA, He′nan Province, Zhengzhou 450042, China
Abstract：Objective To observe the safety of skin medication of herpes ointment. Methods Eight healthy adult New Zealand rabbits were selected and divided into intact skin group and damaged skin group by random number table method, four rabbits in each group. The skin irritation test was carried out by coating herpes ointment and herpes ointment matrix (0.5 g each) with the homologous control method, and the skin was administered for histopathological examination 14 days later. Forty healthy adult guinea pigs were selected and divided into herpes ointment group, matrix group, dinitrochlorobenzene group by random number table method, twenty in herpes ointment group and ten in other groups. The skin sensitization test was carried out by coating herpes ointment (0.2 g each), herpes ointment matrix (0.2 g each), and 2,4-dinitrochlorobenzene (0.2 mL each). Erythema and edema were used as evaluation indexes in two tests. Twenty-four New Zealand rabbits were selected and divided into herpes ointment group, matrix group and blank control group by random number table method. Each group had eight rabbits. Acute skin toxicity tests were carried out on New Zealand rabbits coated with 10 g/kg, 2 g/kg, 2 mL/kg herpes ointment, herpes ointment matrix and normal saline in groups, and the changes of body mass, toxic reaction and death of rabbit in each group were observed within 14 days. On the 14th day after dosing, general anatomy was performed on all New Zealand rabbits, and histopathological examination was performed if there were any toxic reactions and general anatomy abnormalities. Results Herpes ointment had no irritating effect on intact and damaged skin of New Zealand rabbits, and the histopathological examination of skin was normal. Herpes ointment had no skin allergic reaction to guinea pigs, and the sensitization intensity score was 0； there was no acute toxicity reaction to New Zealand rabbits, no obvious abnormalities were found in tissues and organs after dissection, body weight growth was consistent with the growth law. Compared with the blank control group, there was no significant difference in the weight of New Zealand rabbits of the herpes ointment group (P ＞ 0.05). Conclusion Herpes ointment has no skin irritation, allergy, or acute toxicity. The topical skin medication is safe and can be used for clinical applications.
2020, Vol. 17 
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