深海鱼油对应激性胃溃疡的抑制作用

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摘要目的探究深海鱼油对应激性胃溃疡的抑制作用。方法 24只SD大鼠按随机数字表法分为对照组、模型组和鱼油组，每组8只，雌雄各半。鱼油组大鼠连续5 d用深海鱼油0.84 mL/kg灌胃，其他两组大鼠连续5 d用0.9%氯化钠溶液0.84 mL/kg灌胃。三组大鼠实验前1 d禁食不禁水24 h。模型组与鱼油组采用束缚水浸应激法制作大鼠胃溃疡模型。实验结束后测定胃溃疡指数（UI），并进行HE染色，观察胃黏膜受损情况，同时测定血清中超氧化物歧化酶（SOD）活性。结果模型组UI高于对照组；鱼油组UI低于模型组（均P < 0.01）。模型组SOD水平低于对照组；鱼油组SOD水平高于模型组（P < 0.05或P < 0.01）。对照组大鼠的胃黏膜完好无损；模型组及鱼油组大鼠胃黏膜均出现缺损，但鱼油组黏膜缺损仅发生在黏膜浅层，其损伤程度经于模型组。结论深海鱼油对应激性胃溃疡具有一定的抑制效应，其机制可能与提高SOD活性，促进氧自由基的清除有关。

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	王鑫荃1 庞泽星1 吴逸钊1 刘淇兴1 区锶文1 余印林1 王桂房2 许小洋2
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关键词 ：深海鱼油, 应激性胃溃疡, 溃疡指数, 超氧化物歧化酶

Abstract：Objective To explore the inhibitory effect of deep-sea fish oil on stress gastric ulcer. Methods According to the random number table method, 24 SD rats were divided into control group, model group and fish oil group, with 8 rats in each group, half male and half female. Rats in fish oil group were gavaged with 0.84 mL/kg of deep-sea fish oil for 5 d consecutive, and rats in the other two groups were gavaged with 0.84 mL/kg of 0.9% sodium chloride solution for 5 d consecutive. The rats in three groups fasted for 24 h before the experiment. The rats in model group and fish oil group were treated with the shackled water immersion stress to make rat gastric ulcer model. After the end of the experiment, the stomach ulcer index (UI) was measured, and HE staining was performed to observe the damage of gastric mucosa. Meanwhile, the activity of superoxide dismutase (SOD) in serum was measured. Results UI of model group was higher than that of control group; UI of fish oil group was lower than that of model group (all P < 0.01). SOD level in model group was lower than that in control group; SOD level of fish oil group was higher than that of model group (P < 0.05 or P < 0.01). The gastric mucosa of rats in the control group was intact. Defects were found in the gastric mucosa of rats in both model group and fish oil group, but the defect occurred only in the superficial mucosa of fish oil group, and the degree of damage was less than that of model group. Conclusion Deep-sea fish oil has a certain inhibitory effect on stress gastric ulcer, this effect may be mediated by enhancing SOD activity and consequently promoting the ability to scavenge oxygen free radicals.

Key words： Deep-sea fish oil Stress gastric ulcer Ulcer index Superoxide dismutase

基金资助:广东省高等教育教学改革项目（粤教高函〔2018〕180号）；
广东省自然科学基金面上项目（2019A1515010983）；
广州医科大学质量工程项目（广医大发〔2019〕5号）；
广州医科大学大学生实验室开放项目（广医大发〔2018〕27号）。

通讯作者: 许小洋（1971.4-），男，博士，副教授；研究方向：巨噬细胞溶酶体胆固醇调节。

引用本文:

王鑫荃1 庞泽星1 吴逸钊1 刘淇兴1 区锶文1 余印林1 王桂房2 许小洋2,3. 深海鱼油对应激性胃溃疡的抑制作用[J]. 中国医药导报, 2021, 18(2): 8-11.
WANG Xinquan1 PANG Zexing1 WU Yizhao1 LIU Qixing1 OU Siwen1 YU Yinlin1 WANG Guifang2 XU Xiaoyang2,3. Inhibitory effect of deep-sea fish oil on stress gastric ulcer. 中国医药导报, 2021, 18(2): 8-11.

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