信号转导和转录活化因子3和碱性螺旋-环-螺旋转录因子1在卵巢恶性上皮肿瘤中的表达

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摘要目的探讨信号转导和转录活化因子3（STAT3）和碱性螺旋-环-螺旋转录因子1（Twist1）在卵巢恶性上皮肿瘤中的表达，并分析其表达失调与卵巢癌发生和发展的关系。方法收集2015年3月～2016年12月于武汉大学人民医院行手术的49例卵巢恶性上皮肿瘤患者和14例卵巢良性肿瘤患者的组织，应用免疫组化SP法检测STAT3和Twist1蛋白的表达情况，比较其在早期卵巢癌和晚期卵巢癌中的表达差异，并作Pearson相关性分析。结果 STAT3和Twist1在卵巢癌中的阳性表达率明显高于卵巢良性肿瘤，差异有统计学意义（P ＜ 0.05）；二者在晚期卵巢癌患者中的阳性表达率明显高于早期卵巢癌，差异有统计学意义（P ＜ 0.05）；Twist1与STAT3在早期和晚期卵巢癌中的阳性表达均呈正相关（r = 0.653、0.798，均P ＜ 0.05）。结论 STAT3和Twist1在卵巢癌的发生、发展和恶化中发挥着重要作用，其阳性表达率与卵巢癌FIGO分期相关，其机制可能是通过上皮-间质转化实现的。

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	洪莉1▲ 杨将1▲ 邢辉2 卢丹华1 汤剑明1 李秉枢1 郭凤琴1

关键词 ：信号转导和转录活化因子3, 碱性螺旋-环-螺旋转录因子1, 相关性, 卵巢恶性上皮肿瘤

Abstract：Objective To investigate the expression of signal transducer and activators of transcription 3 (STAT3) and Twist basic helix-loop-helix transcription factor 1(Twist1) in epithelial ovarian cancer, and to analyze the relationship between the expression dysregulation and the occurrence and development of ovarian cancer. Methods The tissue of 49 cases with epithelial ovarian cancer and 14 cases with ovarian benign tumor taken surgery in Renmin Hospital of Wuhan University from March 2015 to December 2016 were collected. The expression of STAT3 and Twist1 protein was examined by immunohistochemistry streptavidin peroxidase conjugated method, the expression differences of which in early ovarian cancer and advanced ovarian cancer were compared and analyzed by Pearson correlation analysis. Results The positive expression rates of STAT3 and Twist1 in ovarian cancer were significantly higher than those of ovarian benign tumor, the differences were statistically significant (P ＜ 0.05). The positive expression rates of STAT3 and Twist1 in the advanced ovarian cancer were higher than those of early ovarian cancer, the differences were statistically significant (P ＜ 0.05). The positive expression of Twist1 in early ovarian cancer and advanced ovarian cancer was both positively correlated with STAT3(r = 0.653, 0.798, all P ＜ 0.05). Conclusion The expression of STAT3 and Twist1 plays an important role in the occurrence, development and deterioration of ovarian cancer, the positive expression rate has correlation with FIGO stage of ovarian cancer, and the mechanism may be achieved through epithelial-mesenehymal transition.

Key words： Signal transducer and activators of transcription 3 Twist basic helix-loop-helix transcription factor 1 Correlation Epithelial ovarian cancer

基金资助:湖北省卫生计生西医类重点项目（WJ2015MA 024）；
湖北省医学领军人才培养工程专项经费资助项目。

通讯作者: 邢辉（1969.11-），女，博士，主任医师，教授；研究方向：妇科肿瘤。

作者简介: ▲共同第一作者

引用本文:

洪莉1▲ 杨将1▲ 邢辉2 卢丹华1 汤剑明1 李秉枢1 郭凤琴1. 信号转导和转录活化因子3和碱性螺旋-环-螺旋转录因子1在卵巢恶性上皮肿瘤中的表达[J]. 中国医药导报, 2017, 14(24): 118-121.
HONG Li1▲ YANG Jiang1▲ XING Hui2 LU Danhua1 TANG Jianming1 LI Bingshu1 GUO Fengqin1. Expression of signal transducer and activators of transcription 3 and Twist basic helix-loop-helix transcription factor 1 in epithelial ovarian cancer. 中国医药导报, 2017, 14(24): 118-121.

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