Determination of 7 nucleosides in red yeast rice by HPLC
LI Yongsheng1 ZHANG Chunling1 YANG Yuanyuan2 CHEN Zhiyon1,3
1.Department of Pharmacy, Honghui Hospital, Xi′an Jiaotong University of Medicine School, Shaanxi Province, Xi′an 710054, China;
2.Xi′an Institute for Food and Drug Control, Shaanxi Province, Xi′an 710054, China;
3.Institute of Chinese Materia Medica, Shaanxi Academy of Traditional Chinese Medicine, Shaanxi Province, Xi′an 710003, China
Abstract：Objective To establish a high performance liquid chromatography method for the simultaneous determination of seven nucleosides of uracil, cytidine, hypoxanthine, adenine, inosine, thymidine and adenosine in red yeast rice. Methods Shiseido C18 (4.6 mm×250 mm，5 μm) was adopted to separate the sample at the column temperature of 25°C. Acetonitrile (A)-0.05 mol/L KH2PO4 (B) was used as the mobile phase. Gradient elution method: 0-9 min, 0% A; 9-40 min, 0%-8% A. The injection volume was 20 μL, and flow rate was 1 mL/min. The detection wavelength was 254 nm. Results Uracil, cytidine, hypoxanthine, adenine, inosine, thymidine and adenosine showed a good linear relationship with the peak area (r < 0.9995) in the range of 1.00-5.00 μg, 3.08-15.35 μg, 2.78-13.95 μg, 9.37-46.87 μg, 4.58-22.86 μg, 1.06-5.29 μg, 4.41-22.28 μg, respectively. The average recovery rates were 99.34%, 99.56%, 100.18%, 97.95%, 98.02%, 99.28%, RSD < 2.00%. Conclusion The method is reproducible and accurate, and can be used for the determination and quality evaluation of nucleotide in red yeast rice.
2019, Vol. 16 
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