Molecular mechanism of Mahuang Fuzi Xixin Decoction in the treatment of asthma based on network pharmacology
JIA Menghuan1 ZHANG Cairong2 FENG Longfei3
1.School of Chemical and Biological Engineering, Yichun University, Jiangxi Province, Yichun 336000, China;
2.Departerment of Acupuncture and Moxibustion, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Jiangsu Province, Nanjing 210000, China;
3.School of Cosmetic Medical, Yichun University, Jiangxi Province, Yichun 336000, China
Abstract：Objective To explore the key active components and molecular mechanism of Mahuang Fuzi Xixin Decoction in the treatment of asthma based on network pharmacology. Methods The active components and drug targets of Mahuang Fuzi Xixin Decoction were searched and screened in traditional Chinese medicine systems pharmacology database and analysis platform, and the target was standardized by Uniprot database. The genes related to asthma were retrieved by using comparative toxicogenomics database. The therapeutic target was obtained by intersection of drug target and disease target by Venn diagram. The therapeutic targets were imported into the STRING database for analysis, and the proteinprotein interaction network was constructed. The target greater than the average value of degree, betweenness centrality, and closeness centrality was the key target. Finally, GO enrichment analysis and KEGG signaling pathway analysis were carried out for the key targets. Results The results of literature review and analysis showed that there were five main active ingredients in Mahuang Fuzi Xixin Decoction: quercetin, luteolin, kaempferol, β-sitosterol, and naringenin. The key targets include tumor necrosis factor (TNF), interleukin-6, TP53, etc. KEGG enrichment analysis showed that Mahuang Fuzi Xixin Decoction involved 15 major signaling pathways including TNF signaling pathway and Toll-like receptor signaling pathway. GO enrichment showed that there were 124 biological processes, 24 molecular functions, and 12 cell compositions in the treatment of asthma by Mahuang Fuzi Xixin Decoction. Conclusion Mahuang Fuzi Xixin Decoction in the treatment of asthma may have multi-channel, multi-target, multi-component joint effect, the treatment of asthma provides the basis of bioinformatics.
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