VEGF、Hsa-miR-93、S100A8在乳腺癌中的表达及与预后的关系分析

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摘要目的分析血管内皮细胞生长因子（VEGF）、Hsa-miR-93、S100钙结合蛋白A8（S100A8）在乳腺癌中的表达及与预后的关系。方法选取2017年1月至2018年6月安徽省池州市人民医院治疗并经病理确诊的77例初发乳腺癌患者的癌组织及相应的癌旁正常组织。采用免疫组织化学法检测VEGF、S100A8的表达；原位杂交方法检测组织中Hsa-miR-93的表达；分析VEGF、Hsa-miR-93、S100A8表达水平与患者临床病理特征的关系；根据患者术后3年的生存资料，分析VEGF、Hsa-miR-93、S100A8表达水平对预后生存的影响。结果乳腺癌组织中的VEGF、Hsa-miR-93、S100A8阳性表达率高于癌旁正常组织（P ＜ 0.05）。癌组织中VEGF阳性表达与淋巴结转移有关（P ＜ 0.05），与年龄、组织学分级、TNM分期、肿瘤直径无关（P ＞ 0.05）；癌组织中Hsa-miR-93阳性表达与TNM分期、淋巴结转移有关（P ＜ 0.05），与年龄、组织学分级、肿瘤直径无关（P ＞ 0.05）；癌组织中S100A8阳性表达与组织学分级、TNM分期、淋巴结转移有关（P ＜ 0.05），与年龄、肿瘤直径无关（P ＞ 0.05）。VEGF、Hsa-miR-93、S100A8阳性表达的乳腺癌患者与阴性表达的乳腺患者生存率比较，差异无统计学意义（P ＞ 0.05）。结论 VEGF、miR-93、S100A8在乳腺癌组织中呈高表达，但阳性表达率与生存率无明显差异。

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	徐亮1 王杜娟2 黄述斌1 谢倩1 李松梅1

关键词 ：乳腺癌, 血管内皮细胞生长因子, Hsa-miR-93, S100钙结合蛋白A8, 病理特征, 预后

Abstract：Objective To analyze the expressions of vascular endothelial growth factor (VEGF), Hsa-miR-93, and S100 calcium binding protein A8 (S100A8) in breast cancer and their relationship with prognosis. Methods The cancer tissues and corresponding para-carcinoma nomal tissues were collected from 77 patients pathologically confirmed with new breast cancer who were treated in the Chizhou People’s Hospital from January 2017 to June 2018. The expressions of VEGF and S100A8 in tissues were detected by immunohistochemistry; the expression of Hsa-miR-93 was detected by in situ hybridization method; the relationship between VEGF, Hsa-miR-93, S100A8 levels and clinicopathological characteristics of patients were analyzed; according to patients’ survival data of three years after surgery, the effects of VEGF, HSA-miR-93, and S100A8 expression levels on prognosis and survival were analyzed. Results The positive expression rates of VEGF, Hsa-miR-93, and S100A8 in breast cancer tissues were higher than those in para-carcinoma nomal tissues (P < 0.05). The positive expression of VEGF in cancer tissues was related to lymph node metastasis (P < 0.05), while not related to age, histological grading, TNM staging or tumor diameter (P > 0.05); the positive expression of Hsa-miR-93 in cancer tissues was related to TNM staging and lymph node metastasis (P < 0.05), while not related to age, histological grading or tumor diameter (P > 0.05); the positive expression of S100A8 in cancer tissues was related to histological grading, TNM staging, and lymph node metastasis (P < 0.05), while not related to age or tumor diameter (P > 0.05). There was no significant difference in survival rate between patients with positive expression of VEGF, HSA-miR-93, and S100A8 in breast cancer tissues and patients with negative expression (P > 0.05). Conclusion VEGF, miR-93, and S100A8 are highly expressed in breast cancer tissues, but there is no significant difference in positive expression rate and survival rate.

Key words： Breast cancer Vascular endothelial growth factor Hsa-miR-93 S100 calcium binding protein A8 Pathological characteristic Prognosis

基金资助:广东省基础与应用基础研究基金项目（2019A15 15110188）。

引用本文:

徐亮1 王杜娟2 黄述斌1 谢倩1 李松梅1. VEGF、Hsa-miR-93、S100A8在乳腺癌中的表达及与预后的关系分析[J]. 中国医药导报, 2022, 19(17): 32-36.
XU Liang1 WANG Dujuan2 HUANG Shubin1 XIE Qian1 LI Songmei1. Expressions of VEGF, Hsa-miR-93, and S100A8 in breast cancer and their relationship with prognosis. 中国医药导报, 2022, 19(17): 32-36.

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https://www.yiyaodaobao.com.cn/CN/ 或 https://www.yiyaodaobao.com.cn/CN/Y2022/V19/I17/32

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