Effects of PLGF gene silencing on proliferation, migration, and invasion of human extravillous cytotrophoblasts and Nrf2/HO-1 signaling pathway in high glucose environment
SHU Xiaoming CHEN Xiaoqin SHAO Wen
Department of Obstetrics, the Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, Jiangsu Province, Huaian 223300, China
Abstract：Objective To investigate the effect of placental growth factor (PLGF) gene silencing on human extravillous cytotrophoblasts (EVCT) in high glucose environment. Methods HTR-8/SVneo cells were cultured in vitro, and the cells were divided into control group (no treatment), empty group (empty plasmid transfection) and PLGF silencing group (PLGF double-incision enzyme plasmid transfection). The cell proliferation, migration, and invasion ability of each group were detected by CCK-8 method, scratch test, and Transwell test; the cell mRNA and protein expressions of PLGF, nuclear factor-erythroid 2-related factor 2 (Nrf2), and heme oxygenase-1 (HO-1) in each group were detected by qRT-PCR and Western blot. Results There were no significant differences in cell proliferation inhibition rate, scratch healing rate, and cell invasion number between control group and empty group (P＞0.05); compared with control group, the cell proliferation inhibition rate was increased, the scratch healing rate and the number of invasive cells were decreased in PLGF silencing group (P＜0.05). The mRNA and protein expressions of PLGF, Nrf2, and HO-1 were not significantly different between control group and empty group (P＞0.05); compared with control group, the mRNA and protein expressions of PLGF, Nrf2, and HO-1 in PLGF silencing group were decreased (P＜0.05). Conclusion PLGF gene silencing can inhibit the proliferation, migration, and invasion of EVCT in high glucose environment, and its mechanism may be related to inhibiting the activity of Nrf2/HO-1 signaling pathway.
束晓明 陈晓琴 邵雯. 高糖环境下PLGF基因沉默对人绒毛膜外滋养细胞增殖、迁移及侵袭及Nrf2/HO-1信号通路的影响[J]. 中国医药导报, 2023, 20(28): 26-29.
SHU Xiaoming CHEN Xiaoqin SHAO Wen. Effects of PLGF gene silencing on proliferation, migration, and invasion of human extravillous cytotrophoblasts and Nrf2/HO-1 signaling pathway in high glucose environment. 中国医药导报, 2023, 20(28): 26-29.
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