角质细胞生长因子活性短肽促进糖尿病大鼠创面愈合的实验研究

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摘要目的探讨角质细胞生长因子（KGF）活性多肽对糖尿病大鼠创面愈合的促进作用。方法选择24只健康雌性SD大鼠，腹腔注射链脲佐菌素酸（STZ），制备糖尿病大鼠模型。将24只大鼠随机分成4组，即阴性对照组、KGF阳性对照组、KGF活性短肽1组、KGF活性短肽2组，每组6只。分别于大鼠背部制备直径2 cm的圆形创面，并定期对创面局部注射各组药物。伤后14 d进行拍照，记录创面未愈合面积，计算创面愈合率。采用HE染色观察创面愈合情况。结果成功制备糖尿病大鼠慢性创面模型。伤后14 d，KGF阳性对照组、KGF活性短肽1组和KGF活性短肽2组的创面愈合率与阴性对照组比较，显著升高，差异有高度统计学意义（P ＜ 0.01）；KGF活性短肽1组和KGF活性短肽2组的创面愈合率与KGF阳性对照组比较，差异无统计学意义（P ＞ 0.05）。阴性对照组创面皮肤全层缺失，毛囊、汗腺和皮脂腺等皮肤附件缺失，组织结构均匀致密、无层次，炎症细胞浸润明显。除阴性对照组外，其余各组均创面上皮化良好，深层组织层次清楚、结构疏松，炎症细胞浸润较少，并可见再生的毛囊、汗腺和皮脂腺等皮肤附件。结论 KGF活性短肽可促进糖尿病大鼠的创面愈合及皮肤附件的再生。

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	宗宪磊1 曹春艳1 宋国栋1 赖晨智1 余泮熹1 靳小雷1 姜笃银2
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关键词 ：角质细胞生长因子, 活性短肽, 糖尿病大鼠, 创面愈合

Abstract：Objective To explore the promoting effect of keratinocyte growth factor (KGF) active peptide on wound healing in diabetic rats. Methods Twenty-four healthy female SD rats were selected, and injected intraperitoneally with streptozotocin to prepare a diabetic rat model. They were randomly divided into 4 groups: negative control group, KGF positive control group, No.1 KGF active short peptide group, and No.2 KGF active short peptide group, with 6 rats in each group. Round wounds of 2 cm in diameter were prepared on the back of the rats, and drugs of each group were injected locally on the wounds. Photographs were taken 14 days after injury, and the unhealed area of the wound was recorded to calculate the wound healing rate. The wound healing was observed by HE staining. Results The chronic wound model of diabetic rats was successfully established. At 14 days after injury, the wound healing rate of KGF positive control group, No.1 KGF active short peptide group and No.2 KGF active short peptide group was significantly higher than that of the negative control group, and the difference was highly statistically significant (P < 0.01). The wound healing rate of No.1 KGF active short peptide group and No.2 KGF active short peptide group was not significantly different from that of KGF positive control group (P > 0.05). In the negative control group, the whole skin of the wound was missing, and the skin attachments such as hair follicles, sweat glands and sebaceous glands were missing. The tissue structure was uniform and dense, no layer, and the inflammatory cell infiltration was obvious. In the other groups, the wounds were well formed, the deep tissue layers were clear, the structure was loose, the infiltrated inflammatory cells was lessen, and the skin attachments such as regenerated hair follicles, sweat glands and sebaceous glands were visible. Conclusion KGF active short peptide can promote wound healing and regeneration of skin attachment in diabetic rats.

Key words： Keratinocyte growth factor Bioactive peptide Diabetic rat Wound healing

基金资助:国家自然科学基金资助项目（30670571）；
国家自然科学青年基金资助项目（81201467）；
协和青年科研基金项目（2017310007）。

通讯作者: 靳小雷（1972.3-），男，博士，主任医师；研究方向：颅颌面外科；姜笃银（1963.2-），男，博士，主任医师；研究方向：创面愈合和瘢痕防治，烧伤外科。

作者简介: 宗宪磊（1979.9-），男，博士；研究方向：创面愈合和瘢痕防治，颅颌面外科。

引用本文:

宗宪磊1 曹春艳1 宋国栋1 赖晨智1 余泮熹1 靳小雷1 姜笃银2,3. 角质细胞生长因子活性短肽促进糖尿病大鼠创面愈合的实验研究[J]. 中国医药导报, 2018, 15(30): 4-7.
ZONG Xianlei1 CAO Chunyan1 SONG Guodong1 LAI Chenzhi1 YU Panxi1 JIN Xiaolei1 JIANG Duyin2,3. The experimental study on promoting wound healing of keratinocyte growth factor bioactive peptides in diabetic rats. 中国医药导报, 2018, 15(30): 4-7.

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