Expression of long non-coding RNA tumor protein translationally-controlled regulator 1-antisenseRNA1 in glioma and its effect on cell proliferation and invasion
LIU Zhenjie1 LI Xin1 LU Jing2 LIANG Liang1 HU Yaowen1 XIE Jing1 ZHAO Xingang3 YANG Jiankai4
1.Department of Neurosurgery, Baoding NO.1 Central Hospital, Hebei Province, Baoding 071000, China;
2.Department of Internal Medicine, Baoding Orthopaedic Hospital, Hebei Province, Baoding 071000, China;
3.Department of Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing 100093, China;
4. Department of Neurosurgery, Second Hospital of Hebei Medical University, Hebei Province, Shijiazhuang 050000, China
Abstract：Objective To investigate the effect of long non-coding RNA (lncRNA) tumor protein translation regulator 1-antisense RNA1 (TPT1-AS1) on the proliferation and invasion of glioma cells U251. Methods U251 cells were divided into blank control group, negative transfection group, and TPT1-AS1 overexpression group. The blank control group was not treated, and the other two groups were transfected with corresponding plasmids. After 48 hours of transfection, the expression level of TPT1-AS1 in U251 cells of each group was compared; MTT assay was used to determine the proliferation ability of U251 cells; flow cytometry was used to detect the apoptotic ability of U251 cells; Transwell chamber assay was used to detect the invasion ability of U251 cells; scratch test was used to detect the migration ability of U251 cells; the expression of apoptosis (Caspase-3, Bax, Bcl-2) and invasion (matrix metallo proteinases [MMP]-2, MMP-9) related proteins was detected by Western blot. Results There were no significant differences in TPT1-AS1 expression, cell survival rate, cell apoptosis rate, number of invasive cells, scratch healing rate, and protein expressions of Caspase-3, Bax, Bcl-2, MMP-2, and MMP-9 between negative control group and blank control group (P＞0.05). TPT1-AS1 expression level, apoptosis capacity, Caspase-3, and Bax protein expression in TPT1-AS1 overexpression group were higher than those in blank control group, and the differences were statistically significant (P＜0.05). The expression of Bcl-2 protein, cell survival rate, invasion, migration ability, and invasion-related protein in TPT1-AS1 overexpression group were lower than those in blank control group, with statistical significance (P＜0.05). Conclusion TPT1-AS1 has low expression in U251, and high expression of TPT1-AS1 can inhibit the proliferation and invasion of glioma cells.
刘振杰1 李鑫1 鲁静2 梁良1 胡耀文1 解靖1 赵新岗3 杨建凯4. 长链非编码RNA肿瘤蛋白翻译调节因子1-反义RNA1在神经胶质瘤细胞中的表达及对其增殖和侵袭的影响[J]. 中国医药导报, 2023, 20(4): 25-30.
LIU Zhenjie1 LI Xin1 LU Jing2 LIANG Liang1 HU Yaowen1 XIE Jing1 ZHAO Xingang3 YANG Jiankai4. Expression of long non-coding RNA tumor protein translationally-controlled regulator 1-antisenseRNA1 in glioma and its effect on cell proliferation and invasion. 中国医药导报, 2023, 20(4): 25-30.
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