Expression of long non-coding RNA DEANR1 and others in lung cancer
ZHANG Lei1 HE Yuefeng2 ZHANG Li1 ZHANG Tengfei1 YANG Kaiyun1 HUANG Yunchao1 TAN Hui1
1.The First Department of Thoracic Surgery, the Third Affiliated Hospital of Kunming Medical University, Yunnan Province, Kunming 650500, China;
2.School of Public Health, Kunming Medical University, Yunnan Province, Kunming 650500, China
Abstract：Objective To investigate the expression of long non-coding RNA (lncRNA) DEANR1, INXS, ANCR, FENDRR in lung cancer. Methods The study included 54 patients with lung cancer admitted to the Third Affiliated Hospital of Kunming Medical University from January to June 2018, including squamous cell carcinoma, adenocarcinoma, and small cell lung cancer in 28, 22, 4 cases, respectively. The cancer tissue, the lymph node metastasis and the tissue adjacent to carcinoma of 54 cases of patients with lung cancer was used to detect the expression of these genes by the real-time fluorescence quantitative PCR. Results The expression levels of INXS and ANCR in cancer tissues were higher than those in adjacent normal tissues (P ＜ 0.05). The expression levels of ANCR and INXS in lymph node tissues were positively correlated with ki-67 (r = 0.757, P = 0.049; r = 0.802, P = 0.030). Conclusion The increased expression of DEANR1, INXS and ANCR in cancer tissues may be a positive factor in the development of lung cancer. The expression of ANCR and INXS in lymph nodes is positively correlated with ki-67, which may be related to lymph node metastasis and poor prognosis of lung cancer.
[1] Ponting CP，Oliver PL，Reik W. Evolution and functions of long noncoding RNAs [J]. Cell，2009，136（4）：629-641.
[2] Gibb EA，Vucic EA，Enfield KS，et al. Human cancer long non-coding RNA transcriptomes [J]. PLoS One，2011，6（10）：e25915.
[3] Taft RJ，Pang KC，Mercer TR，et al. Non-coding RNAs：regulators of disease [J]. J Pathol，2010，220（2）：126-139．
[4] Silva JM，Perez DS，Pritchett JR，et al. Identification of long stress-induced non-coding transcripts that have altered expression in cancer [J]. Genomics，2010，95（6）：355-362.
[5] Jiang W，Liu Y，Liu R，et al. The lncRNA DEANR1 facilitates human endoderm differentiation by activating FOXA2 expression [J]. Cell Rep，2015，11（1）：137-148.
[6] DeOcesano-Pereira C，Amaral MS，Parreira KS，et al. Long non-coding RNA INXS is a critical mediator of BCL-XS induced apoptosis [J]. Nucleic Acids Res，2014，42（13）：8343-8355.
[7] Li ZW，Dong MC，Fan DG，et al. LncRNA ANCR down-regulation promotes TGF-β-induced EMT and metastasis in breast cancer [J]. Oncotarget，2017，8（40）：67329-67343.
[8] 徐然，尚超，石文君.长链非编码RNA FENDRR对非小细胞肺癌细胞增殖及凋亡的影响[J].现代肿瘤医学，2016，24（17）：2667-2669.
[9] Wapinski O，Chang HY. Long noncoding RNAs and human disease [J]. Trends Cell Biol，2011，21（6）：354-361.
[10] Zhang F，Peng H. LncRNA-ANCR regulates the cell growth of osteosarcoma by interacting with EZH2 and affecting the expression of p21 and p27 [J]. J Orthop Surg Res，2017， 12（1）：103.
[11] Yang ZY，Yang F，Zhang YL，et al. LncRNA-ANCR down-regulation suppresses invasion and migration of colorectal cancer cells by regulating EZH2 expression [J]. Cancer Biomark，2017，18（1）：95-104.
[12] 王旭晖，王嵩，田铁栓，等.Ki67、bcl-2、bax蛋白在非小细胞肺癌中的表达及预后价值[J].首都医科大学学报，2005，26（6）：744-746.
[13] 张莉萍，侯立坤，谢惠康，等.P63、P40、CK5/6在小细胞肺癌中的表达及其意义[J].中华病理学杂志，2015，44（9）：644-647.
[14] Taheri ZM，Mehrafza M，Mohammadi F，et al. The diagnostic value of Ki-67 and repp86 in distinguishing between benign and malignant mesothelial proliferations [J]. Arch Pathol Lab Med，2008，132（4）：694-697.
[15] 徐晓艳，杜华，宝鲁日，等.非小细胞肺癌活检标本中CK7、TTF-1、NapsinA、CK5/6及p63的表达及其意义[J].诊断病理学杂志，2015，22（11）：688-691.
[16] Koren A，Sodja E，Rijavec M，et al. Prognostic value of cytokeratin-7 mRNA expression in peripheral whole blood of advanced lung adeno-carcinoma patients [J]. Cell Oncol（Dordr），2015，38（5）：387-395.
2019, Vol. 16 
京公网安备 11010502046598号